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Human Bronchial Epithelial Cells: HBEpC



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Top Left: Human Bronchial Epithelial Cells, HBEpC
Top Right: HBEpC immunofluorescently stained with anti-CK18 antibody
Bottom Left/Right: HBEpC transfected with GFP plasmid DNA using the Cytofect™ Epithelial Cell Transfection Kit.

Human Bronchial Epithelial Cells, HBEpC, are derived from the surface epithelium of normal human bronchi. They are cryopreserved at first passage and can be cultured and propagated at least 16 population doublings. Airway epithelial cells are targets in asthma, viral infection, and fibrotic lung disease. These cells have been used to study the regulation of intracellular pH1, the stimulant effect of IL-1b on airway epithelial cell growth2, and the effect of human rhinovirus infection in terms of upregulation of ICAM-1 expression on epithelial cells3.

  1. Poulsen, J.H. et al, J Physiol.-Abs. Vol. 432 (3) (1996).
  2. Murlas, C.G. et al, Lung 175: 117-126 (1997).
  3. Terajima, M. et al, Lung cellular and molec. physiology, 273 (4):749-759 (1997).
 Characterization: Typical epithelial cell morphology.
 
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Surface epithelium of normal bronchi
Cryopreserved ampoule:
1st passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved Human Bronchial Epithelial Cells (502-05a), 500 ml of Bronchial/Tracheal Epithelial Cell Growth Medium (511-500), (511-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Growth Medium at 2nd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings

Cytofect Epithelial Cell Transfection Kit
Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HBEpC, adult Each ampoule contains >500,000 cryopreserved cells. Each kit contains one ampoule, 500 ml Growth Medium (511-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		502K-05a
502-05a 		$795
$646
Proliferating HBEpC, adult Cultured and shipped at second passage in Bronchial/Tracheal Epithelial Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		503-25a
503-75a
503-6Wa
503-96Wa 		$646
$836
$836
$956
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Bronchial/Tracheal Epithelial Cell Growth Medium Kit Basal Medium (BM) & Growth Supplement (GS) are bottled separately but packaged together in the Growth Medium Kit. Basal Medium and Growth Supplement are mixed together to yield 500 ml Growth Medium. 500 ml
5 ml 		510-500
511-GS 		$58
$58
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Bronchial/Tracheal Epithelial Cell Growth Medium Optimized and ready for use. 500 ml 511-500 $108
Bronchial/Tracheal Epithelial Cell Growth Medium Kit Basal Medium (BM, Cat.# 510-500) & Growth Supplement (GS, Cat.# 511-GS) are bottled separately but packaged together in the Growth Medium Kit. Basal Medium and Growth Supplement are mixed together to yield 500 ml Growth Medium. Kit 511K-500 $116
Bronchial/Tracheal Epithelial Cell Growth Medium Kit Basal Medium (BM) & Growth Supplement (GS) are bottled separately but packaged together in the Growth Medium Kit. Basal Medium and Growth Supplement are mixed together to yield 500 ml Growth Medium. 500 ml
5 ml 		510-500
511-GS 		$58
$58
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
HBEpC RNA, adult Total RNA prepared from HBEpC 10 ug
25 ug 		502-R10a
502-R25a 		$325
$650
 
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