|
|
Human Peripheral Blood Monocytes (HPBM) are the precursors of tissue macrophages and dentritic cells. Circulating monocytes descend from self-renewing hematopoietic stem cells that initiate myeloid differentiation and give rise to multipotent precursors. Monocytes are central to a number of immunological responses, including cell-mediated cytotoxicity and production of inflammatory mediators. In humans, circulating monocytes are divided into two subsets on the basis of the expression of CD14, a component of the lipopolysaccharide (LPS) receptor complex, and CD16, the FcγRIII immunoglobulin receptor. These monocyte subsets express distinct chemokine, immunoglobulin, adhesion, and scavenger receptors. CD14+ monocytes are large with diameter of ~18 μm, represent ~80%–90% of circulating monocytes, and express higher levels of CD62L (L-selectin) and CD11b (also referred to as Mac-1 or CR3) and lower levels of MHC class II than do CD16+ monocytes.1 CD14+ and CD16+ monocytes respond to distinct trafficking cues; CD14+ monocytes express high levels of CCR1, CCR2, and CXCR2 and low levels of CX3CR1, and thus respond to monocyte chemoattractant protein MCP-1 (CCL2), whereas CD16+ monocytes express high levels of CX3CR1 and low levels of CCR2, and respond to fractalkine (CX3CL1) in transendothelial migration assays.
In the setting of infection, monocytes contribute directly to immune defense against microbial pathogens.2 Recent investigations have started to reveal how the constitutive pathway of monocyte maturation and differentiation into tissue macrophages and dendritic cells. Regulation of T cell responses by monocytes in the setting of microbial infection is complex, with positive and negative contributions to T cell proliferation and differentiation.3 Moreover, monocytes can be activated via a number of intracellular signaling pathways (eg, c-Jun amino terminal kinase, p38 mitogen-activated protein kinase, FcR/Syk, Janus kinase/signal transducer and activator of transcription) that induce production of mediators of inflammation.4 Thus, targeting selected monocyte/macrophage signaling pathways is a potential therapeutic strategy to suppress monocyte-mediated tissue injury
Human Peripheral Blood CD14+ Monocytes (HPBM) should provide a useful tool for studying various aspects of pathology and biology of the human monocyte/macrophage/dentritic cells in vitro. To meet various needs for research and development, Cell Applications, Inc isolated and purified Human Peripheral Blood CD14+ Monocytes (HPBM) to create a more realistic in vitro model. Cell Applications' Human Peripheral Blood CD14+ Monocytes (HPBM) are isolated and purified from human peripheral blood of healthy donors and cryopreserved immediately after isolation.
1. Passlick, B. et al, Blood 74:2527 (1989)
2. Taylor, P.R. et al, Immunity 19:2 (2003)
3. Serbina, N.V. et a, Immunity 19:59 (2003)
4. Ma, F.Y. et al, Semin. Nephrol. 30:334 (2010)
Characterization:
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
|
 |
|
Tissue:
|
Normal human peripheral blood
|
|
Cryopreserved ampoule:
|
Immediately after isolation, 5,000,000 cells in DMEM containing 45% FBS & 10% DMSO.
|
|
Kit contains:
|
Ampoule of cryopreserved HPBM (6906-50a), 250 ml Blood Cell Maintenance Medium (615-250)
|
|
Cells in flasks or mulitwell plates:
|
Shipped in Blood Cell Transfer Medium in either flasks or multiwell plates.
|
|
Population doublings:
|
Blood cells do no proliferate in culture
|
|
