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Human Renal Proximal Tubular Epithelial Cells: HRPTEpC



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Left: Human Renal Proximal Tubular Epithelial Cells, HRPTEpC
Right: HRPTEpC immunofluorescently stained with anti-CK19

The proximal tubule is the portion of the duct system of the nephron of the kidney which leads from Bowman's capsule to the loop of Henle. It has a single epithelial cell layer. The luminal surface of the epithelial cells of the proximal tubule is bordered with millions of microvilli that form a brush border and increase surface area. The basal and lateral cell membrane forms infoldings in a complex labyrinth in contact with the interstitial fluid and blood vessels.1 HRPTEpCs have a pivotal role in kidney functions such as reabsorbtion of glucose and protein filtered by glomerulus and secretion of organic acids and drugs; they also play a part in the glucotoxicity associated with diabetes.2,3  The progression of renal dysfunction in type 2 diabetes heavily involves injury of the tubular epithelium as well as the glomerulus.4

It has been shown that under normal circumstances tubular epithelial cells in the adult rat kidney turn over very little, but after an acute injury many mitotic cells have been observed both by proliferating cell nuclear antigen (PCNA) staining and by incorporation of the thymidine analogue bromodeoxyuridine (BrdU). Alternative explanations have been proposed such as the existence of resident renal stem cells or the influx of hematopoietic stem cells from the bone marrow5 instead of the initial belief that the surviving epithelial cells dedifferentiate, move through the cell cycle until tubular integrity is restored, go back into G0 phase, and redifferentiate.

Human Renal Proximal Tubular Epithelial Cells (HRPTEpC) provide a useful tool for studying various aspects of pathology and biology of  the human renal proximal tubular cells in vitro. To meet various needs for research and development, Cell Applications, Inc isolated and purified HRPTEpC to create a more realistic in vitro model. Cell Applications' HRPTEpC are isolated from proximal tubules of human kidney. They are cryopreserved at second passage and can be cultured and propagated at least 3 population doublings for adult HRPTEpC and 10 population doublings for neonatal HRPTEpC.  The cells have a characteristic morphology consistent with an epithelial origin and are positive for epithelial cell marker cytokeratin 19
. 
 
1. Weier, Q.I. et al, Nephrol. 12:155 (2007)
2. Gilbert R. E. et al, Kidney Int 56:1627 (1999)
3. Ziyadeh F. N. et al, Am. J Physiol. 259:F704 (1990)
4. Debnam E. S. et al, Kidney Int 50:110 (1996)
5. Vogetseder, A. et al, Am. J. Physiol. Cell Physiol. 292:C807(2007)
 
Characterization:  
 
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal human bladder
Cryopreserved ampoule:
First or second passage, >500,000 cells in RenaEpi Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HRPTEpC (930-05), 500 ml RenaEpi Growth Medium (911-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in RenaEpi Cell Transfer Medium at third passage in either flasks or multiwell plates.
Population doublings:
Can be cultured at least 3-11 doublings


Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HRPTEpC, adult Contains an ampoule of cryopreserved HRPTEpC (930-05), 500 ml RenaEpi Growth Medium (911-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule
930K-05a
930-05a
$770
$619
Proliferating HRPTEpC, adult Cultured and shipped in RenaEpi Transfer Medium at third passage in either flasks or multiwell plates. T-25 Flask
T-75 Flask
6 well
96 well
931-25a
931-75a
931-6Wa
931-96Wa
$619
$809
$809
$929
Cryopreserved HRPTEpC, fetal Contains an ampoule of cryopreserved HRPTEpC (930-05), 500 ml RenaEpi Growth Medium (911-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule
930K-05f
930-05f
$780
$629
Proliferating HRPTEpC, fetal Cultured and shipped in RenaEpi Transfer Medium at third passage in either flasks or multiwell plates. T-25 Flask
T-75 Flask
6 well
96 well
931-25f
931-75f
931-6Wf
931-96Wf
$629
$819
$819
$939
Cryopreserved HRPTEpC, neonatal Contains an ampoule of cryopreserved HRPTEpC (930-05), 500 ml RenaEpi Growth Medium (911-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule
930K-05n
930-05n
$780
$629
Proliferating HRPTEpC, neonatal Cultured and shipped in RenaEpi Transfer Medium at third passage in either flasks or multiwell plates. T-25 Flask
T-75 Flask
6 well
96 well
931-25n
931-75n
931-6Wn
931-96Wn
$629
$819
$819
$939
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
RenaEpi Growth Medium Optimized and ready for use 500 ml 911-500 $115
RenaEpi Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 911K-500 $125
RenaEpi Basal Medium Add Growth Supplement before use 500 ml 910-500 $60
RenaEpi Growth Supplement Added to Basal Medium to create Growth Medium 5 ml (3 parts) 911-GS $65