BACKGROUND Transfection, the introduction of DNA into cells, is a powerful technique used to study in vivo gene function and regulation. Cytofect-Fibroblast Transfection kit is a plasmid DNA delivery system specifically optimized to deliver DNA into a wide variety of fibroblasts. This kit contains Cytofect 2, Enhancer and Media for transfection and culturing cells. Peptide Enhancer complexes with DNA and Cytofect 2 and escorts the tranfection complex to the nucleus. The result has been high transfection efficiency and viability for these traditionally hard-to-transfect primary fibroblasts.
This Fibroblast Transfection Kit has successfully transfected the following primary fibroblasts with high efficiency (30%-70%) and viability (90%-95%):
HDF: Human Dermal Fibroblasts
HCF: Human Cardiac Fibroblasts
HLF: Human Lung Fibroblasts
Products are for research use only. They are not intended for human, animal, or diagnostic applications. TR2 and PE are manufactured by Targeting Systems.