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Rat Osteoblasts: ROb



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Rat Osteoblasts, ROb

Rat Osteoblasts (ROb) are isolated from rat bone and are cryopreserved at second passage. ROb can be passaged at least 10 population doublings. ROb are a powerful tissue engineering tool for the study of signaling pathways and 3-D modeling. Sustained delivery of basic fibroblast growth factor (bFGF) enhances bone formation following osteoblast transplantation1. Prostaglandin E increases fibronectin and promotes bone formation in ROb via the EP(1) receptor, phospholipase C, PKCalpha, and c-SRC signaling pathway2. Via the FGF2 pathway, prostaglandin F2-alpha regulates ROb metabolism3. Osteoblasts express A disintegrin and metalloprotease-1 (ADAMTS-1), which breaks down extracellular matrix molecules and is expressed at regions of new bone formation4. ADAMTS-1 is a target for parathyroid hormone in bone5. Hypoxia decreases the growth, differentiation and bone-forming capacity of ROb6. ROb can be used in tissue engineering models. Rat osteoblasts have been cultured in 3-D matrices, including processed bovine cancellous bone which resulted in osteoblast differentiation7. Plasma-treated porous polylactide membranes increases the attachment and growth of ROb for the use of tissue engineering8.
  1. Park, MS et al., J Biomec Mater Res B Appl Biomater 79(2):353 (2006).
  2. Tang, CH et al., J Biol Chem 280(24):22907 (2005).
  3. Marchetti, L et al., J Cell Biochem 97(6):1379 (2006).
  4. Lind, T e tl., Bone. 36(3):408 (2005).
  5. Miles, RR et et al., Endocrinology. 141(12):4533 (2000).
  6. Utting, JC et al., Exp Cell Res. 312(10):1693 (2006).
  7. Stangenberg, L et al., Tissue Eng. 11(5-6):855 (2005).
  8. Gugala, Z & Gogolewski, S, J Biomed Mater Res A. 76(2):288 (2006).
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Rat bone
Cryopreserved ampoule:
1st passage, >500,000 cells in Rat Osteoblast Basal Medium that contains 10% FBS and 10% DMSO.
Kit contains:
Ampoule of cryopreserved ROb (R406-05a), 500 ml of Rat Osteoblast Growth Medium (R417-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Rat Osteoblast Growth Medium at 2nd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 10 population doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved ROb Each kit contains an ampoule of >500,000 cryopreserved cells, 500 ml of Rat Osteoblast Growth Medium (R417-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		R406K-05a
R406-05a 		$738
$595
Proliferating ROb Cultured and shipped at second passage in Rat Osteoblast Growth Medium. T-25 flask
T-75 flask
6 well
96 well 		R407-25a
R407-75a
R407-6Wa
R407-96Wa 		$595
$785
$785
$905
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Rat Osteoblast Growth Medium Optimized and ready for use. 500 ml R417-500 $102
Rat Osteoblast Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500ml R417K-500 $110
Rat Osteoblast Basal Medium Add Growth Supplements before use. 500 ml R416-500 $58
Rat Osteoblast Growth Supplements Added to Basal Medium to create Growth Medium 50 ml R417-GS $53
Rat Osteoblast Differentiation Medium Rat Osteoblast Differentiation Medium 250 ml R417D-250 $63
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51