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Rat Neural Stem Cells: RNSC



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A: Rat Neural Stem Cells, RNSC. B: Differentiated RNSC immunofluorescently stained with anti-B-tubulin. C: Differentiated RNSC immunofluorescently stained with anti-GFAP.

Rat Neural Stem Cells (RNSC) are isolated from cerebral striatum and mesencephalon of E18 fetal or adult rat brains. These cells were maintained in undifferentiated proliferative state by culturing them as free floating neurospheres in serum-free medium optimized with growth factors. They are cryopreserved at first passage and can be cultured and propagated for 1-2 passages1 prior to induction of phenotypic differentiation. These stem cells are multipotent2,3  and can be induced to differentiate into neurons, astrocytes and oligodendrocytes that make up the central nervous system (CNS). Neurospheres transplanted into intact brain can survive, expand and differentiate into mature neurons, astrocytes and oligodendrocytes precursors4 in 3 weeks. The ability of neural stem cells to retain multi-lineage potential and proliferate extensively in vitro provides new avenues for the treatment of neural degenerative diseases and injuries. 
  1. Svendsen, C.N. et al, Brain Res. Dev. 99:253 (1997).
  2. Reynolds, B.A. et al, J. Neuroscience 12(11):4565 (1992).
  3. Reynolds, B.A. et al, Develop. Bio. 175:1 (1996).
  4. Karbanova, J. et al. Biomed. Papers 148(2):217 (2004).

Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
E18 fetal or adult rat brain cerebral striatum and mesencephalon
Cryopreserved ampoule:
1st passage, >2 million cells in Rat Neural Stem Cell Basal Medium that contains 10% FBS and 10% DMSO.
Kit contains:
Ampoule of cryopreserved RNSC (RS820-20f), 250 ml of Rat Neural Stem Cell Growth Medium (R813-250).
Proliferating Cells:
 
Shipped in Rat Neural Stem Cell Growth Medium at 2nd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured 1-2 passages

 


Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved RNSC, fetal Each kit contains an ampoule of >2 million cryopreserved fetal RNSC, 250 ml of Rat Neural Stem Cell Growth Medium (R813-250). 1 Kit
1 Ampoule
RS820K-20f
RS820-20f
$665
$565
Proliferating RNSC, fetal Cultured and shipped at second passage in Rat Neural Stem Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well
RS821-25f
RS821-75f
RS821-6Wf
RS821-96Wf
$565
$755
$755
$875
 
Media and Regeants
Product Name Product Description Size Cat. No. Price
Rat Neural Stem Cell Growth Medium All-in-one ready-to-use media 250 ml R813-250 $110
Rat Neural Stem Cell Growth Medium Kit Basal Medium and Growth Supplement sold together, bottled separately yields 250 ml R813K-250 $119
Rat Neural Stem Cell Basal Medium Growth Medium without Growth Supplement 250 ml R812-250 $37
Rat Neural Stem Cell Growth Supplements Added to Basal Medium to create Growth Medium 10 ml R813-GS $82
Rat Neural Stem Cell Differentiation Medium Rat Neural Stem Cell Differentiation Medium 250 ml R813D-250 $100
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51