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Rat Lung Fibroblasts: RLF



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Rat Lung Fibroblasts, RLF

Rat Lung Fibroblasts (RLF) are derived from the lung of adult Sprague Dawley rat. The cells are cryopreserved at second passage. RLF can be cultured and propagated 10 population doublings. It was found that TGF-beta and PDGF produced by stimulated macrophages are involved in the regulation of the expression of alpha1(1) collagen, of prolyl-4-hydroxylase and lysyloxidase in rat lung fibroblasts1. Antisense calcyclin (S100A6) RNA expression was found to inhibit serum and mechanical strain induced fibroblasts proleferation2. Activation of fibroblasts by TGF-beta(1) by interferon-gamma may have potential therapeutic benefits in bleomycin-induced pulmonary fibrosis3. Furthermore, sulfated proteoglycans play a key role in modulating pulmonary fibroblast extracellular matrix composition and are important mediators of elastolytic injury4, and addition of heparin-binding EGF-like growth factor to pulmonary fibroblasts increases FGF-2 mRNA and protein levels.
  1. Koslowski, R. et al, Exp. Toxicol Patholo. 2003 Nov; 55(4): 257.
  2. Breen EC, Tang K, J Cell Biochem, 2003 Mar 1; 88(4): 848.
  3. Venkatesan, N. et al Am J Physiol Lung Cell Mol Physiol. 2002 Oct 283(4): L806.
  4. Buczek-Thomas JA, et al, J Cell Physiol. 2002 Sep; 192(3): 294.
  5. Liu, J, et al, Am J Physiol Lung Cell Mol Physiolo. 2003 Nov; 285(5):L1106.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Sprague Dawley rat lung
Cryopreserved ampoule:
2nd passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO.
Kit contains:
Ampoule of cryopreserved RLFa (R506-05a) or RLFn (R506-05n), 500 ml of Rat Fibroblast Growth Medium (R116-500), a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Rat Fibroblast Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 10 population doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved RLF, adult Each kit contains an ampoule of >500,000 cryopreserved cells, 500 ml of Rat Fibroblast Growth Medium (R116-500), a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		R506K-05a
R506-05a 		$708
$575
Proliferating RLF, adult Cultured and shipped at third passage in Rat Fibroblast Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		R507-25a
R507-75a
R507-6Wa
R507-96Wa 		$575
$765
$765
$885
Cryopreserved RLF, neonatal Each kit contains an ampoule of >500,000 cryopreserved cells, 500 ml of Rat Fibroblast Growth Medium (R116-500), a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		R506K-05n
R506-05n 		$708
$575
Proliferating RLF, neonatal Cultured and shipped at third passage in Rat Fibroblast Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		R507-25n
R507-75n
R507-6Wn
R507-96Wn 		$575
$765
$765
$885
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Rat Fibroblast Growth Medium Optimized and ready for use 500 ml R116-500 $92
Rat Fibroblast Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml R116K-500 $100
Rat Fibroblast Basal Medium Add Growth Supplement before use 500 ml R115-500 $53
Rat Fibroblast Growth Supplement Added to Basal Medium to create Growth Medium 50 ml R116-GS $47
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51