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Rat Hepatocytes: RH



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Rat Hepatocytes, RH

Rat Hepatocytes (RH) are isolated from adult rat livers. They are cryopreserved at the end of primary cell isolation. The regulatory effect of insulin-like growth factor-11 on hepatocyte function and the apoptotic effect of transforming growth factor-b2 were examined in primary hepatocytes. As liver transplantation tends to cause hypoxia, rat hepatocytes serve as a model in investigating liver pathophysiology3. Rat hepatocytes have been used in a wide variety of clinical experiments such as drug metabolism, toxicology4, and liver tissue engineering.
  1. Herrera, B. et al, FEBS Lett. 499(3)225-229 (2001). 
  2. Skrtic, S. et al, Horm. Res. 55 Suppl. 1:1-16 (2001). 
  3. Kaibori, M. et al, Life Sci 69(1): 17-26 (2001). 
  4. Mankiewicz, J. et al, Environ. Toxicol. 16(3):225-233 (2001).
Products are for research use only. They are not intended for human, animal, or diagnostic applications. 

Tissue:
Adult rat liver
Cryopreserved ampoule:
Primary isolation, >3,000,000 cells in Rat Hepatocyte Maintenance Medium that contains 10% FBS and 10% DMSO.
Kit contains:
Ampoule of cryopreserved RH (R780-30n), 250 ml of Rat Hepatocyte Maintenance Medium (R713-250) for Hepatocyte Suspension kit. The Matrigel Adherent Hepatocyte Kit contains: 100 ml of Rat Hepatocyte Attachment Medium (R711-100), & 2.5 ml of Matrigel (126-2.75). The Collagen Adherent Hepatocyte Kit contains: 100 ml of Rat Hepatocyte Attachment Medium (R711-100), & Rat Tail Collagen Coating Solution (122-20).
Cultured Cells: 
Shipped in Maintenance Medium in either flasks or multiwell dishes.
Population doublings:
n/a

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved RH (Suspension) Each Rat Hepatocyte in Suspension Total Kit contains an ampoule of >3,000,000 cryopreserved RH (R780-30) and 250 ml of Rat Hepatocyte Maintenance Medium (R713-250). 1 Kit
1 Ampoule 		R780K-30s
R780-30n 		$398
$350
Cryopreserved RH (Matrigel) Each Rat Hepatocyte Adherent in Matrigel Total Kit contains an ampoule of >3,000,000 cryopreserved RH (R780-30), 2.75 ml of Matrigel (126-2.75), 100 ml of Rat Hepatocyte Attachment Medium (R711-100), and 250 ml of Rat Hepatocyte Maintenance Medium (R713-250). 1 Kit R780K-30m $554
Cryopreserved RH (Collagen) Each Rat Hepatocyte Adherent in Collagen Total Kit contains an ampoule of >3,000,000 cryopreserved RH (R780-30), Rat Tail Collagen Solution (124-20), 100 ml of Rat Hepatocyte Attachment Medium (R711-100), and 250 ml of Rat Hepatocyte Maintenance Medium (R713-250). 1 Kit R780K-30c $465
Cultured RH Shipped in Maintenance Medium in either flasks or multiwell dishes. C: Collagen, M: Matrigel T-25 Flask
T-75 Flask
6 well
96 well
T-25 Flask
T-75 Flask
6 well
96 well 		R781c-25n
R781c-75n
R781c-6Wn
R781c-96Wn
R781m-25n
R781m-75n
R781m-6Wn
R781m-96Wn 		$350
$540
$540
$660
$350
$540
$540
$660
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Rat Hepatocyte Attachment Basal Medium Optimized and ready for use. 100 ml R710-100 $37
Rat Hepatocyte Attachment Medium Optimized and ready for use. 100 ml R711-100 $42
Rat Tail Collagen Coating Solution Optimized and ready for use. 20 ml 122-20 $25
Matrigel    2.5 ml 126-2.5 $114
Rat Hepatocyte Maintenance Basal Medium Optimized and ready for use. 250 ml R712-250 $50
Rat Hepatocyte Maintenance Medium Optimized and ready for use. 250 ml R713-250 $58