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Rat Dermal Fibroblasts: RDF



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Rat Dermal Fibroblasts, RDF

Rat Dermal Fibroblasts (RDF) are derived from the dermis of normal Sprague Dawley rats. They are cryopreserved at first passage and can be cultured and propagated at least 8 population doublings. The arginase activity was significantly elevated in wound–derived fibroblasts from Lewis rats compared to normal dermal fibroblasts underlines the distinct regulation of l-arginine metabolism in wound-derived fibroblasts1. RDF were modified genetically to act like Schwann’s cells to deliver nerve growth factor2. The role of skin fibroblast elastase on the degeneration of elastic fiber caused by UV irradiation was tested on the rat hind leg3.
  1. Witte, M.B. et al, J. Surg. Res. 105(1):35-42 (2002).
  2. Patrick, C.W. et al, Ann. Plast. Surg. 47(6):660-5 (2001).
  3. Tsukahara, K. et al, J. Invest. Dermatol. 117(3):671-7 (2001).
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
 

Tissue:
Sprague Dawley rat dermis
Cryopreserved ampoule:
1st passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO.
Kit contains:
Ampoule of cryopreserved RDF (R106-05n), 500 ml of Rat Fibroblast Growth Medium (R116-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Rat Fibroblast Growth Medium at 2nd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 8 population doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved RDF Each kit contains an ampoule of >500,000 cryopreserved cells, 500 ml of Rat Fibroblast Growth Medium (R116-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		R106K-05n
R106-05n 		$608
$475
Proliferating RDF Cultured and shipped at second passage in Rat Fibroblast Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		R107-25n
R107-75n
R107-6Wn
R107-96Wn 		$475
$665
$665
$785
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Rat Fibroblast Growth Medium Optimized and ready for use. 500 ml R116-500 $92
Rat Fibroblast Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml R116K-500 $100
Rat Fibroblast Basal Medium Add Growth Supplement before use 500 ml R115-500 $53
Rat Fibroblast Growth Supplement Added to Basal Medium to create Growth Medium 50 ml R116-GS $47
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51