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Rat Cardiac Fibroblasts: RCF



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Rat Cardiac Fibroblasts, RCF

Rat Cardiac Fibroblasts (RCF) are derived from the heart tissue of normal Sprague Dawley rats. They are cryopreserved at second passage and can be cultured and propagated at least 8 population doublings. Rat Cardiac Fibroblasts are known to play key roles in the development of cardiac disease and hypertrophy1. The regulation of fibroblast growth after acute myocardial infarction was determined in RCF2. Cardiac fibroblasts were examined as a potential source of pro-inflammatory cytokine IL-6 synthesis which had been implicated in the pathophysiology of heart disease3. RCF have also been used to study the autocrine/paracrine capability mediated by vasodilator such as adernomedullin4. RCF are used to investigate their role in local hormone metabolism5 . Nitric oxide (NO) production in RCF decreases cAMP accumulation largely by the cGMP-mediated activation of PDE26 .  IL-1 beta-induced inducible NO synthase expression can trigger NO-dependent apoptosis in adult RCF7.
  1. Mareinfeld, U. et al, Basic Res. Cardiolo. 96(2):184 (2001)
  2. Staufenberger, S. et al, J. Cell. Physiol. 187(3):326 (2001)
  3. Burger, A. et al, Am. J. Physiol. Circ. Physiol. 281(1):H14 (2001)
  4. Tomoda, Y. et al, Cariosac Res. 49(4):721 (2001)
  5. van der Hides, S.M. et al, J. Endocrinol. 174(1):111 (2002).
  6. Gustafsson, A.B., Brunton, L.L. Am. J. Physiol. Cell Physiol. 283(2):C463 (2002).
  7. Tian, B. et al, Am. J. Physiol. Heart Circ. Physiol. 283(5):H1958 (2002). 
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Neonatal Sprague Dawley rat heart tissue
Cryopreserved ampoule:
2nd passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO.
Kit contains:
Ampoule of cryopreserved RCFa (R306-05a) or RCFn (R306-05n), 500 ml of Rat Fibroblast Growth Medium (R116-500), and a Subculture Reagent Kit (090K).
Cells:
 
Shipped within 3 days after plating in Rat Cardiomyocyte Culture Medium in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 8 population doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved RCF, adult Each kit contains an ampoule of cryopreserved RCF (R306-05), 500 ml of Rat Fibroblast Growth Medium (R116-500), and a Subculture Reagent Kit. 1 Kit
1 Ampoule 		R306K-05a
R306-05a 		$728
$595
Proliferating RCF, adult Cultured and shipped at third passage in Rat Fibroblast Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		R307-25a
R307-75a
R307-6Wa
R307-96Wa 		$595
$785
$785
$905
Cryopreserved RCF, neonatal Each kit contains an ampoule of cryopreserved RCF (R306-05), 500 ml of Rat Fibroblast Growth Medium (R116-500), and a Subculture Reagent Kit. 1 Kit
1 Ampoule 		R306K-05n
R306-05n 		$728
$595
Proliferating RCF, neonatal Cultured and shipped at third passage in Rat Fibroblast Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		R307-25n
R307-75n
R307-6Wn
R307-96Wn 		$595
$785
$785
$905
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Rat Fibroblast Growth Medium All-in-one ready-to-use media 500 ml R116-500 $92
Rat Fibroblast Growth Medium Kit Basal Medium and Growth Supplement sold together, bottled separately yields 500 ml R116K-500 $100
Rat Fibroblast Basal Medium Growth Medium without Growth Supplement 500 ml R115-500 $53
Rat Fibroblast Growth Supplement Added to Basal Medium to create Growth Medium 50 ml R116-GS $47
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51