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Rat Brain Microvascular Endothelial Cells: RBMVEC



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Left: Rat Brain Microvascular Endothelial Cells, RBMVEC. Right: RBMVEC stained for ZO-1, with DAPI nuclear counterstain. (Courtesy of Dr. Robert Pearlstein and Maria Moldovan of Loma Linda University/ Radiation Medicine)

Rat Brain Microvascular Endothelial Cells (RBMVEC) are derived from the brain of adult Sprague Dawley rat. The cells are cryopreserved at second passage. RBMVEC can be cultured and propagated 16 population doublings. Endothelial cell-derived monocyte-chemoattractant protein-1 (MCP-1) plays a key role in leukocyte recruitment across the blood-brain and blood-retinal barriers in vivo.1 Three-dimensional model indicates that dipyridamole can improve BBB Function.2 Mainteance of BBB function during and following normoxic-cormoglycemic flow cessation appears to depend on intact nitric oxide signaling and IL-6 release.3 Expression of metabotropic glutamate receptors may modulate synaptic transmission and determine microvascular function and dysfunction.4 VEGF mRNA and VEGF receptor F1K-1/KDR are both higher in neonatal than in adult microvessels suggesting that microvascular cell turnover continues in the adult brain.5 Hypoxia increases the aparacellualr flux across the cell monolayer via the release of VEGF, which in turn leads to the dislocalization, decreased expression and enhanced phosphorylation of Zonula occludens-1 (ZO-1).6
  1. Harkness, KA, et al, Neuroimmunol. 2003 Sep; 142(1-2):1.
  2. Parkinson FE, et al, Brain Res. 2003 Aug. 8; 980(2) 233.
  3. Krizanac-Bengez, L, et al, Brain Res. 2003 Jul. 11; 977(2): 239.
  4. Gillard, SE, et al, J Comp. Neurol. 2003 Jun. 30; 461(3): 317.
  5. Hoehn, BD, et al, Brain Res. Mol Brain Res. 2002 May 30; 101(1-2): 103.
  6. Fischer S. et al, Microvasc Res. 2002 Jan; 63(1): 70.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Sprague Dawley adult rat brain
Cryopreserved ampoule:
2nd passage, >500,000 cells in Rat Brain Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.
Kit contains:
Ampoule of cryopreserved   RBMVEC (R840-05a), 500 ml of Rat Brain Endothelial Cell Growth Medium (R819-500), 100 ml of Attachment Factor Solution (123-100), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Rat Brain Endothelial Cell Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 population doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved RBMVEC Each kit contains an ampoule of >500,000 cryopreserved cells, 500 ml of Rat Brain Endothelial Cell Growth Medium (R819-500), 100 ml of Attachment Factor Solution (123-100), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		R840K-05a
R840-05a 		$904
$725
Proliferating RBMVEC Cultured and shipped at third passage in Rat Brain Endothelial Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		R841-25a
R841-75a
R841-6Wa
R841-96Wa 		$725
$915
$915
$1035
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Rat Brain Endothelial Cell Growth Medium Optimized and ready for use. 500 ml R819-500 $120
Rat Brain Endothelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500 ml R819K-500 $128
Rat Brain Endothelial Cell Basal Medium Add Growth Supplement before use. 500 ml R818-500 $53
Rat Brain Endothelial Cell Growth Supplement Added to Basal Medium to create Growth Medium 50 ml R819-GS $75
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
RBMVEC RNA Total RNA prepared from RBMVEC 10 ug
25 ug 		R840-R10a
R840-R25a 		$380
$760