|
Rat Brain Microvascular Endothelial Cells (RBMVEC) are derived from the brain of adult Sprague Dawley rat. The cells are cryopreserved at second passage. RBMVEC can be cultured and propagated 16 population doublings. HIV-1 Tat protein alters the tight junction protein expression and distribution in cultured brain endothelial cells. It is believed such alteration lead to disturbances of the blood-brain barrier (BBB) integrity and contributes to HIV trafficking in to the brain1. Endothelial cell-derived monocyte-chemoattractant protein-1 (MCP-1) plays a key role in leukocyte recruitment across the blood-brain and blood-retinal barriers in vivo2 . Three-dimensional model indicates that dipyridamole can improve BBB Function3. Mainteance of BBB function during and following normoxic-cormoglycemic flow cessation appears to depend on intact nitric oxide signaling and IL-6 release4. Expression of metabotropic glutamate receptors may modulate synaptic transmission and determine microvascular function and dysfunction5. VEGF mRNA and VEGF receptor F1K-1/KDR are both higher in neonatal than in adult microvessels suggesting that microvascular cell turnover continues in the adult brain6. Hypoxia increases the aparacellualr flux across the cell monolayer via the release of VEGF, which in turn leads to the dislocalization, decreased expression and enhanced phosphorylation of Zonula occludens-1 (ZO-1)7.
-
Andras IE, et al, J Neurosci Res. 2003 Oct 15; 74(2): 255.
-
Harkness, KA, et al, Neuroimmunol. 2003 Sep; 142(1-2):1.
-
Parkinson FE, et al, Brain Res. 2003 Aug. 8; 980(2) 233.
-
Krizanac-Bengez, L, et al, Brain Res. 2003 Jul. 11; 977(2): 239.
-
Gillard, SE, et al, J Comp. Neurol. 2003 Jun. 30; 461(3): 317.
-
Hoehn, BD, et al, Brain Res. Mol Brain Res. 2002 May 30; 101(1-2): 103.
-
Fischer S. et al, Microvasc Res. 2002 Jan; 63(1): 70.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
|
 |
|
Tissue:
|
Sprague Dawley adult rat brain
|
|
Cryopreserved ampoule:
|
2nd passage, >500,000 cells in Rat Brain Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.
|
|
Kit contains:
|
Ampoule of cryopreserved RBMVEC (R840-05), 500 ml of Rat Brain Endothelial Cell Growth Medium (R819-500), 100 ml of Attachment Factor Solution (123-100), and a Subculture Reagent Kit (090K).
|
|
Proliferating Cells:
|
Shipped in Rat Brain Endothelial Cell Growth Medium at 3rd passage in either flasks or multiwell dishes.
|
|
Population doublings:
|
Can be cultured at least 16 population doublings
|
- Publications utilizing CAI RBMVEC
- RBMVEC Instruction Sheet
- RBMVEC FAQs
- Related products
|
