Main Page >> >> Rat Aortic Endothelial Cells: RAOEC
Rat Aortic Endothelial Cells: RAOEC



(Enlarge Image)

A: Rat Aortic Endothelial Cells, RAOEC. B&C: RAOEC transfected with GFP plasmid DNA using the Cytofect™ Endothelial Cell Transfection Kit.

Rat Aortic Endothelial Cells (RAOEC) are isolated from aorta of healthy adult rats. They are cryopreserved at third passage and can be cultured and propagated to sixth passage and beyond without losing their morphologic and phenotypic characteristics. RAOEC is a relevant in vitro model for correlating with in vivo studies in rat as an animal model. RAOEC have been used as a model for the study of Atherosclerosis1 and products synthesized by the endothelium2,3. The interaction of aortic endothelium and smooth muscle cells regulate the level and distribution of glycosaminoglycans in the arterial wall, especially in the intima4. Co-culture of aortic endothelial cells and smooth muscle cells provide an ideal in vitro model for studying the interaction between these two cells types.
  1. Ross, R., Nature 362:801 (1993)
  2. Falcy, C., et. al., Collagen Rel. Res. 5:519 (1985)
  3. McGuire, P.G., et.al., Microcirc.Endo.Lymph. 2:129 (1985)
  4. Merrilees, M.J., et.al., Atherosclerosis 39:147 (1981)
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Adult rat aorta
Cryopreserved ampoule:
3rd passage, >500,000 cells in Rat Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.
Kit contains:
Ampoule of cryopreserved  RAOEC (R304-05a), 500 ml of Rat Endothelial Cell Growth Medium (R211-500), 100 ml of Attachment Factor Solution (123-100) and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Rat Endothelial Cell Growth Medium at 4th passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least to 6th passage

Cytofect Endothelial Cell Transfection Kit
Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved RAOEC Each kit contains an ampoule of >500,000 cryopreserved cells, 500 ml of Rat Endothelial Cell Growth Medium (R211-500), 100 ml of Attachment Factor Solution (123-100), and a Subculture Reagent Kit. 1 Kit
1 Ampoule 		R304K-05a
R304-05a 		$892
$725
Proliferating RAOEC Cultured and shipped at fourth passage in Rat Endothelial Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		R305-25a
R305-75a
R305-6Wa
R305-96Wa 		$725
$915
$915
$1035
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Rat Endothelial Cell Growth Medium Optimized and ready for use 500 ml R211-500 $102
Rat Endothelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml R211K-500 $110
Rat Endothelial Cell Basal Medium Add Growth Supplement before use 500 ml R210-500 $55
Rat Endothelial Cell Growth Supplement Added to Basal Medium to create Growth Medium 15 ml R211-GS $55
Attachment Factor Solution Promotes attachment of endothelial cells. 100 ml 123-100 $18
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
RAOEC RNA Total RNA prepared from RAOEC 10 ug
25 ug 		R304-R10a
R304-R25a 		$380
$760