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PCASMC: Porcine Coronary Artery Smooth Muscle Cells



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Porcine Coronary Artery Smooth Muscle Cells, PCASMC

Porcine Coronary Artery Smooth Muscle Cells (PCASMC) are derived from tunica intima and tunica media of healthy, fibrous plaque-free porcine coronary arteries. They are cryopreserved at second passage and can be cultured and propagated at least population 14 doublings. Smooth muscle cells have been found in fatty streaks of early arteriosclerosis. Proliferation of the smooth muscle cells is considered a key event in the development of advanced lesions1. Porcine smooth muscle cells serve as an alternative for the investigations of cardiac disease and arteriosclerosis1-3. It can be used in co-culture investigation with species-matched porcine artery endothelial cells.
  1. Ross, R.N., Nature 362:801 (1993).
  2. Stary, H.C., Arteriosclerosis 3:471a (1983).
  3. McGill, H.C. Jr., Arteriosclerosis 4:443 (1984).
Animal tissues were USDA inspected.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
 

Tissue:
Normal porcine coronary artery
Cryopreserved ampoule:
2nd passage, >500,000 cells in Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.
Kit contains:
Ampoule of cryopreserved PCASMC (P350-05), 500 ml of Porcine Smooth Muscle Cell Growth Medium (P311-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Porcine Smooth Muscle Cell Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved PCASMC Each kit contains an ampoule of >500,000 cryopreserved cells, 500 ml Porcine Smooth Muscle Cell Growth Medium, and a Subculture Reagent Kit (090K). 1 Kit
1 Ampuole 		P350K-05
P350-05 		$664
$525
Proliferating PCASMC Cultured and shipped in flasks at third passage in Porcine Smooth Muscle Cell Growth Medium. T-25 flask
T-75 flask
6 well
96 well 		P351-25
P351-75
P351-6w
P351-96w 		$525
$715
$715
$835
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Porcine Smooth Muscle Cell Growth Medium All-in-one ready-to-use media 500 ml P311-500 $98
Porcine Smooth Muscle Cell Growth Medium Kit Basal Growth Medium and Growth Supplement sold together, bottled separately yields 500 ml P311K-500* $105
Porcine Smooth Muscle Cell Basal Medium Growth Medium without Growth Supplement 500 ml P310-500 $53
Porcine Smooth Muscle Cell Growth Supplement Added to Basal Medium to create Growth Medium 50 ml P311-GS $53
Porcine Smooth Muscle Cell Induction Medium Porcine Smooth Muscle Cell Induction Medium 250 ml P311I-250 $58
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51