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MNSC: Mouse Neural Stem Cells



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A: Mouse Neural Stem Cells, MNSC. B: Differentiated MNSC immunofluorescently stained with anti-B-tubulin. C: Differentiated MNSC immunofluorescently stained with anti-GFAP.

Mouse Neural Stem Cells (MNSC) are isolated from cerebral striatum and mesencephalon of E14 fetal mouse brains. These cells were maintained in an undifferentiated proliferative state by culturing them as free-floating neurospheres in serum-free medium optimized with growth factors. MNSC are cryopreserved at second passage and can be cultured and propagated for 1-2 passages prior to induction of phenotypic differentiation. These stem cells are multipotent1,2 and can be induced to differentiate into neurons, astrocytes and oligodendrocytes. Neural stem cells are a source of donor tissue for prospective neural cell therapy given the ability of neurosphere to retain multi-lineage potential and proliferative ability in vitro.
  1. Reynolds, B.A. et al, J. Neuroscience 12(11):4565 (1992).
  2. Reynolds, B.A. et al, Develop. Bio. 175:1 (1996).
Characterization: Differentiated mouse neural stem cells are positive for β-tubulin III, GFAP, and oligodendrocyte marker O4. Each lot undergoes a bioassay in which the ability to form neurospheres in Mouse Neural Stem Cell Growth Medium is tested.
 
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Mouse E14 fetal brain cerebral striatum and mesencephalon
Cryopreserved ampoule:
1st passage, >2 million cells in Mouse Neural Stem Cell Basal Medium that contains 10% FBS and 10% DMSO.
Kit contains:
Ampoule of cryopreserved MNSC (MS820-20f), 250 ml of Mouse Neural Stem Cell Growth Medium (M813-250), and two 10 cm non-tissue culture treated dishes.
Proliferating Cells:
 
Shipped in Mouse Neural Stem Cell Growth Medium at 2nd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured 1-2 passages

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved MNSCf Each kit contains an ampoule of >2 million cryopreserved cells, 250 ml of Mouse Neural Stem Cell Growth Medium (M813-250), and two 10 cm non-tissue culture treated dishes. 1 Kit
1 Ampoule 		MS820K-20f
MS820-20f 		$759
$659
Proliferating MNSCf Cultured and shipped at second passage in Mouse Neural Stem Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		MS821-25f
MS821-75f
MS821-6Wf
MS821-96Wf 		$659
$849
$849
$969
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
MNSC Growth Medium Optimized and ready for use . 250 ml M813-250 $110
MNSC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 250 ml M813K-250 $119
MNSC Basal Medium Add Growth Supplement before use 250 ml M812-250 $37
MNSC Growth Supplement Added to Basal Medium to create Growth Medium 10 ml M813-GS $82
Mouse Neural Stem Cell Differentiation Medium Mouse Neural Stem Cell Differentiation Medium 250 ml M813D-250 $63