Mouse Neural Stem Cells (MNSC) are isolated from cerebral striatum and mesencephalon of E14 fetal mouse brains. These cells were maintained in an undifferentiated proliferative state by culturing them as free-floating neurospheres in serum-free medium optimized with growth factors. MNSC are cryopreserved at second passage and can be cultured and propagated for 1-2 passages prior to induction of phenotypic differentiation. These stem cells are multipotent1,2 and can be induced to differentiate into neurons, astrocytes and oligodendrocytes. Neural stem cells are a source of donor tissue for prospective neural cell therapy given the ability of neurosphere to retain multi-lineage potential and proliferative ability in vitro.
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Characterization: Differentiated mouse neural stem cells are positive for β-tubulin III, GFAP, and oligodendrocyte marker O4. Each lot undergoes a bioassay in which the ability to form neurospheres in Mouse Neural Stem Cell Growth Medium is tested.
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