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CnSkMC: Canine Skeletal Muscle Cells



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Canine Skeletal Muscle Cells, CnSkMC

Canine Skeletal Muscle Cells (CnSkMC) are derived from the skeletal muscle of canine limb and are cryopreserved at second passage. CnSkMC can be passaged at least 15 population doublings. Muscle diseases can occur in dogs with clinical presentations resembling human disorders, thus the canine system can be a valuable intermediate disease model1,2. The canine model has been used to study the effects of hypoxia3,4,5, ischemia6,7, and hemorrhagic shock8 on skeletal muscle. CnSkMC represent an in vitro cellular system to study the effects of diet on skeletal muscle9,10,11.
  1. Shelton, GD & Enqvall, E, Neuromuscul Disord. 15(2):127 (2005).
  2. Ytterberg, SR, Curr Opin Rheumatol. 3(6):934 (1991).
  3. Mo, FM & Ballard, HJ. Am J Physiol Heart Circ Physiol 289(4):H1710 (2005).
  4. Mo, FM & Ballard, HJ. J Physiol. 536(2):592 (2001).
  5. da Cruz, CA et al., J Cardiovasc Surg (Torino). 38(5):473 (1997).
  6. Timmons, JA et al., J Clin Invest. 97(3):879 (1996).
  7. Roberts, PA et al., J Physiol. 544(2):591 (2002).
  8. Sunder-Plassman, L et al., Res Exp Med (Berl) 159(3):167 (1973).
  9. McKenzie, E et al., Med Sci Sports Exerc. 37(8(:1307 (2005).
  10. Helman, EE et al., J Anim Sci. 81(9):2199 (2003).
  11. Reynolds, Aj et al., J Appl Physiol. 79(5):1601 (1995).
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal skeletal muscle of the canine limb
Cryopreserved ampoule:
1st passage, >500,000 cells in Canine Skeletal Muscle Cell Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved CnSkMC (Cn150-05), 500 ml Canine Skeletal Muscle Cell Growth Medium (Cn151-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Canine Skeletal Muscle Cell Growth Medium at 2nd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 15 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved CnSkMC Each kit contains an ampoule of >500,000 cryopreserved cells, 500 ml of Canine Skeletal Muscle Cell Growth Medium (Cn151-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		Cn150K-05
Cn150-05 		$746
$595
Proliferating CnSkMC Cultured in flasks at second passage in Canine Skeletal Muscle Cell Growth Medium. T-25 flask
T-75 flask
6 well plate
96 well plate 		Cn151-25
Cn151-75
Cn151-6w
Cn151-96w 		$595
$785
$785
$905
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Canine Skeletal Muscle Cell Growth Medium Optimized and ready for use. 500 ml Cn151-500 $110
Canine Skeletal Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500ml Cn151K-500 $119
Canine Skeletal Muscle Cell Basal Medium Add Growth Supplements before use. 500 ml Cn150-500 $56
Canine Skeletal Muscle Cell Growth Supplements Added to Basal Medium to create Growth Medium 50 ml (2 parts) Cn151-GS $63
Canine Skeletal Muscle Cell Differentiation Medium Promotes differentiation of skeletal muscle cells 250 ml Cn151D-250 $58
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51