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CnPASMC: Canine Pulmonary Artery Smooth Muscle Cells



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Canine Pulmonary Artery Smooth Muscle Cells, CnPASMC

Canine Pulmonary Artery Smooth Muscle Cells (CnPASMC) are derived from the tunica intima and tunica media of canine pulmonary arteries and are cryopreserved at second passage. CnAOEC can be passaged at least 16 population doublings. The canine model has been used to study the role of calcium entry1,2,3,4,5,6,7, ClC-3 channels8,9, PDGF10,11, and PKC12,13 on pulmonary artery smooth muscle cell function.
  1. Shimizu, S et al., Anesthesiology. 104(4):791 (2006).
  2. Ng, LC et al., J Physiol. 563(2):409 (2004).
  3. Horibe, M et al., Anesthesiology. 95(3):681 (2001).
  4. Doi, S et al., Am J Physiolo Lung Cell Mol Physiol. 279(2):L242 (2000).
  5. Hong, SJ et al., Anesthesiology. (88)3:792 (1998).
  6. Hamada, H et al., Circ Res. 81(5):812 (1997).
  7. Hamada, H et al., Anesthesiology. 87(4):900 (1997).
  8. Dai, YP et al., Br J Pharmacol. 145(1):5 (2005).
  9. Wang, GX et al., Am J Physiol Heart Circ Physiol. 285(4):H1453 (2003).
  10. Bongalon, S et al., J Vas Res. 41(5):412 (2004).
  11. Yamboliev, IA & Gerthoffer, WT., Am J Physiol Cell Physiol. 280(6): C1680 (2001).
  12. Zhong, J et al., Am J Physiol Cell Physiol. 283(6):C1627 (2002).
  13. Damron, DS et al., Am J Physiol. 274(2pt1):L278 (1998).
Products are for research use only. They are not intended for human, animal, or diagnostic applications. 

Tissue:
Tunica intima and media of normal canine pulmonary artery
Cryopreserved ampoule:
2nd passage, >500,000 cells in Canine Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved CnPASMC (Cn352-05), 500 ml Canine Smooth Muscle Cell Growth Medium (Cn311-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
Shipped in Canine Smooth Muscle Cell Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved CnPASMC Each kit contains an ampoule of >500,000 cryopreserved cells, 500 ml of Canine Smooth Muscle Cell Growth Medium (Cn311-500), and a Subculture Reagent Kit (090K). 1 kit
1 ampoule 		Cn352K-05
Cn352-05 		$728
$595
Proliferating CnPASMC Cultured and shipped at third passage in Canine Smooth Muscle Cell Growth Medium. T-25 flask
T-75 flask
6 well plate
96 well plate 		Cn353-25
Cn353-75
Cn353-6w
Cn353-96w 		$595
$785
$785
$905
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Canine Smooth Muscle Cell Growth Medium Optimized and ready for use. 500 ml Cn311-500 $92
Canine Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500ml Cn311K-500 $100
Canine Smooth Muscle Cell Medium Add Growth Supplements before use. 500 ml Cn310-500 $53
Canine Smooth Muscle Cell Growth Supplements Added to Basal Medium to create Growth Medium 50 ml Cn311-GS $47
Canine Smooth Muscle Cell Induction Medium Canine Smooth Muscle Cell Induction Medium 250 ml Cn311I-250 $63
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51