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CnOb: Canine Osteoblasts



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Canine Osteoblasts, CnOb

Canine Osteoblasts (CnOb) are isolated from canine bone and are cryopreserved at second passage. CnOb can be passaged at least 10 population doublings. The dog model provides a system for the study of bone remodeling agents such as osteoblast-stimulating bone morphogenetic proteins (BMPs)1,2 and bisphosphonates3, as well as anti-remodeling agents4. Canine osteoblasts have been used in prospective bone regeneration models utilzing such 3-D bioactive scaffolds as polycaprolactone5. 
  1. Schmoekel, HG et al., J Orthop Res. 22(2):376 (2004).
  2. ten Dijke, P. Curr Med Res Opin. 22(S):S7 (2006).
  3. Im, GI et al., Biomaterials. 25(18):4105 (2004).
  4. Allen, MR et al., Calcif Tissue Int. 79(4):255 (2006).
  5. Rai, B et al., Biomaterials. 25(24):5499 (2004).
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal canine bone
Cryopreserved ampoule:
1st passage, >500,000 cells in Canine Osteoblast Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved CnOb (Cn406-05), 500 ml Canine Canine Osteoblast Growth Medium (Cn417-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Canine Osteoblast Growth Medium at 2nd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 10 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved CnOb Each kit contains an ampoule of >500,000 cryopreserved cells, 500 ml of Canine Osteoblast Growth Medium (Cn417-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		Cn406K-05
Cn406-05 		$744
$595
Proliferating CnOb Cultured and shipped at second passage in Canine Osteoblast Growth Medium. T-25 Flask
T-75 Flask
6 well plate
96 well plate 		Cn407-25
Cn407-75
Cn407-6w
Cn407-96w 		$595
$785
$785
$905
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Canine Osteoblast Growth Medium Optimized and ready for use. 500 ml Cn417-500 $108
Canine Osteoblast Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500ml Cn417K-500 $116
Canine Osteoblast Basal Medium Add Growth Supplements before use. 500 ml Cn416-500 $58
Canine Osteoblast Growth Supplements Added to Basal Medium to create Growth Medium 30 ml Cn417-GS $58
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
Canine Osteoblast Differentiation Medium Canine Osteoblast Differentiation Medium 250 ml Cn417D-250 $74