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CnAOSMC: Canine Aortic Smooth Muscle Cells



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Canine Aortic Smooth Muscle Cells, CnAOSMC

Canine Aortic Smooth Muscle Cells (CnAOSMC) are derived from tunica intima and tunica media of normal canine aorta. They are cryopreserved at second passage and can be cultured and propagated at least 10 population doublings. Platelet-derived growth factor (PDGF) is a potent mitogen and chemotactic agent which may be involved in intimal hyperplasia and atherosclerosis. It was shown that significant regional difference in PDGF production in normal canine aorta, and that SMC are a significant contributor to the regional variation in PDGF production1. It was also shown that nebivolol relaxes vascular smooth muscle by NO- and cyclic GMP-dependent mechanisms2. Graft SMCs are functionally altered producing more platelet-derived growth factor (PDGF) than aortic SMCs. PDGF produced by graft SMCs may contribute to the development of intimal hyperplasia3. Graft SMCs have decreased proliferation response, but have similar migratory response to PDGF compared with aortic SMCs4. Intrinsic differences in endothelial cells from proximal aorta versus the distal aorta have different capacity to produce PDGF in response to stimulants whereas unstimulated SMCs did not exhibit regional variation in PDGF production and did not increase PDGF secretion after PMA or thrombin teatment5. Identification of the cDNA for canine isoform of nitric oxide synthase (iNOS) can be used in the study of allograft rejection and cardiovascular disease6.

  1. Ignarro, L.J. et al, Nitric Oxide. 7(2):75-82 (2002).
  2. Madura, J.A. et al, J. Vas Res. 33(1):53-61 (1996).
  3. Pitsch, R.J. et al, J. Vasc. Surg. 26(1):70-8 (1997).
  4. Minion, D.J. et al, J. Vasc. Surg. 31(5):953-9 (2000).
  5. Van Aalst, J.A. et al, J. Vasc. Surg. 32(3):584-92 (2000).
  6. Wang, X. et al, Am. J. Physiol. 275(4 Pt 2):H1122-9 (1998).
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Tunica intima and media of normal canine aorta
Cryopreserved ampoule:
2nd passage, >500,000 cells in Canine Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved CnAOSMC (Cn354-05), 500 ml Canine Smooth Muscle Cell Growth Medium (Cn311-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Canine Smooth Muscle Cell Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 10 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved CnAOSMC Each kit contains an ampoule of >500,000 cryopreserved cells, 500 ml of Canine Smooth Muscle Cell Growth Medium (Cn311-500), and a Subculture Reagent Kit. 1 Kit
1 Ampoule 		Cn354K-05
Cn354-05 		$728
$595
Proliferating CnAOSMC Cultured and shipped at third passage in Canine SMC Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		Cn355-25
Cn355-75
Cn355-6W
Cn355-96W 		$595
$785
$785
$905
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Canine Smooth Muscle Cell Growth Medium Optimized and ready for use 500 ml Cn311-500 $92
Canine Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml Cn311K-500 $100
Canine Smooth Muscle Cell Basal Medium Add Growth Supplement before use 500 ml Cn310-500 $53
Canine Smooth Muscle Cell Growth Supplement Added to Basal Medium to create Growth Medium 50 ml Cn311-GS $47
Canine Smooth Muscle Cell Induction Medium Canine Smooth Muscle Cell Induction Medium 250 ml Cn311I-250 $63
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51