Bovine Coronary Artery Smooth Muscle Cells: BCASMC. Isolated from the vessels from which the heart receives its own blood supply.
Bovine Coronary Artery Smooth Muscle Cells: BCASMC
Bovine Coronary Artery Smooth Muscle Cells (BCASMC) are primary smooth muscle cells isolated from bovine coronary arteries.
Description
Bovine Coronary Artery Smooth Muscle Cells (BCASMC) are primary smooth muscle cells isolated from bovine coronary arteries, providing a well-characterized experimental model for studying vascular smooth muscle biology, arterial wall mechanics, and cardiovascular tissue engineering.
In vivo, vascular smooth muscle cells populate the tunica media of large arteries, where they regulate vascular tone, blood pressure, and structural remodeling through contraction and extracellular matrix production. In experimental research, BCASMC — alongside human counterparts like Human Coronary Artery Smooth Muscle Cells (HCASMC) — can be used to investigate cellular responses relevant to cardiovascular disease and atherosclerosis. Characterization protocols for these cells typically include evaluating smooth muscle cell-specific alpha-actin expression, which is used to verify identity and smooth muscle enrichment. Furthermore, BCASMC serve as a valuable platform in biomedical engineering to test novel biomaterials, such as photocrosslinked biodegradable elastomer material surfaces, for their suitability in smooth muscle tissue engineering.
Maintaining experimental reproducibility with BCASMC requires careful adherence to standard cell culture mammalian practices. Cultures are typically handled across controlled passage numbers using standard growth media formulations, which are often formulated without phenol red for assays where phenol red can interfere (colorimetric/fluorescence-based assays). Proper storage temperature management — including cryopreservation protocols utilizing cryoprotectants like DMSO — helps preserve cell viability and functionality. Analyzing downstream experimental results, evaluating gene expression profiles, and utilizing specific antibodies help researchers accurately assess smooth muscle differentiation, phenotype switching, and biochemical and physiological actions under in vitro conditions.
Bovine Coronary Artery Smooth Muscle Cells (BCASMC) from Cell Applications, Inc. have been used to test novel photocrosslinked biodegradable elastomers based material surfaces for their usefulness in smooth muscle tissue engineering.
Characterization: positive for smooth muscle cell specific alpha-actin expression
Details
| Tissue | Normal healthy bovine coronary artery | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma | |
| Character | Smooth muscle specific α-actin positive | |
| Bioassay | Attach, spread, proliferate in Growth Med | |
| Cryovial | 500,000 BCASMC (2nd psg) frozen in Basal Medium w/ 10% FBS, 10% DMSO | |
| Kit | Cryovial frozen BCASMC (B350-05), Growth Medium (B311-500), Subcltr Rgnt Kit (090K) | |
| Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) | |
| Doublings | At least 16 | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
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