Rat Neural Stem Cells (RNSC) are maintained in undifferentiated proliferative state by culturing them as free floating neurospheres in serum-free medium optimized with growth factors. They are cryopreserved at first passage and can be cultured and propagated for 1-2 passages prior to induction of phenotypic differentiation. These stem cells are multipotent and can be induced to differentiate into neurons, astrocytes and oligodendrocytes that make up the central nervous system (CNS). The ability of neural stem cells to retain multi-lineage potential and proliferate extensively in vitro provides new avenues for the treatment of neural degenerative diseases and injuries.
Characterization: differentiated neural stem cells are positive for β-tubulin III, GFAP and oligodendrocyte marker O4.
E18 fetal or adult rat brain cerebral striatum and mesencephalon. Each lot is tested negative for mycoplasma, bacteria, and fungi.
1st passage, >2 million cells in Rat Neural Stem Cell Basal Medium that contains 10% FBS and 10% DMSO.
Ampoule of cryopreserved RNSC (RS820-20f), 250 ml of Rat Neural Stem Cell Growth Medium (R813-250).
Shipped in Rat Neural Stem Cell Growth Medium at 2nd passage in either flasks or multiwell dishes.
Can be cultured 1-2 passages