Left: Human Renal Proximal Tubular Epithelial Cells, HRPTEpC
Right: HRPTEpC immunofluorescently stained with anti-CK19
The proximal tubule is the portion of the duct system of the nephron of the kidney which leads from Bowman\'s capsule to the loop of Henle. It has a single epithelial cell layer. The luminal surface of the epithelial cells of the proximal tubule is covered with millions of microvilli that form a brush border and increase surface area. HRPTEpCs have a pivotal role in kidney function including reabsorbtion of glucose and protein, and also play a part in the glucotoxicity associated with diabetes.
Human Renal Proximal Tubular Epithelial Cells (HRPTEpC) provide a useful tool for studying various aspects of pathology and biology of the human renal proximal tubular cells in vitro. HRPTEpC from Cell Applications, Inc. have recently been used in a study of metabolic enzymes responding to energy depletion in renal cells (Jackson, 2014).
Characterization: the cells have a characteristic morphology consistent with an epithelial origin and are positive for epithelial cell marker cytokeratin 18.
Normal human kidney. Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
First or second passage, >500,000 cells in RenaEpi Basal Medium containing 10% FBS & 10% DMSO.
Ampoule of cryopreserved HRPTEpC (930-05), 500 ml RenaEpi Growth Medium (911-500), and a Subculture Reagent Kit (090K).
Shipped in RenaEpi Cell Transfer Medium at third passage in either flasks or multiwell plates.
Can be cultured at least 3 population doublings for adult HRPTEpC and 10 population doublings for neonatal HRPTEpC
Jackson, E.K., D.G. Gillespie, Z. Mi, D. Cheng, R. Bansal, K. Janesko-Feldman, and P.M. Kochanek. 2014. Role of 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase in the Renal 2',3'-cAMP-Adenosine Pathway. Am J Physiol Renal Physiol: 10.1152/ajprenal.00134.02014.