MSDS Cryopreserved Cells
Instructions HPrEpC
Cell Apps Flyer Epithelial Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Prostate Epithelial Cells (HPrEpC) are isolated from normal human prostate and provide a useful tool for studying various aspects of pathology and biology of the human prostate epithelium in vitro.
HPrEpC and Human Mammary Epithelial Cells (HMEpC), both from Cell Applications, Inc., have been used in a study demonstrating that TGF-b1 induces Smad 1/5/8 and Smad 2/3 phosphorylation and BMP signaling results only in Smad 1/5/8 phosphorylation in these primary epithelial cells, while in cancer cells BMP also elicits Smad2/3 activation, contributing to cancerogenesis.
Characterization: Morphology consistent with epithelial origin, and positive for epithelial cell marker cytokeratin 18

(Click to Enlarge) Human Prostate Epithelial Cells: HPrEpC (L).  HPrEpC immunolabeled for cytokeratin 18 (R).

Details

Tissue
Normal healthy human prostate
QC
No bacteria, yeast, fungi, mycoplasma, virus
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HPrEpC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HPrEpC (934-05a), Growth Medium (215-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Tsfr Med, psg 3, flasks or plates
Doublings
At least 12
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HPrEpC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved Human Prostate Epithelial Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 934K-05a Price: $927.00
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Cryopreserved Prostate Epithelial Cells (HPrEpC), adult: Frozen HPrEpC (5x10^5) Size: 1 Cryovial CAT.#: 934-05a Price: $750.00
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Proliferating Prostate Epithelial Cells (HPrEpC), adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 935-25a Price: $750.00
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Proliferating Prostate Epithelial Cells (HPrEpC), adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 935-75a Price: $940.00
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Proliferating Prostate Epithelial Cells (HPrEpC), adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 935-96Wa Price: $1,060.00
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Related Products

Product Size CAT.# Price Quantity
Human Epi Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 214-500 Price: $84.00
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Human Epi Growth Medium for HPrEpC: All-in-one ready-to-use Size: 500 ml CAT.#: 215-500 Price: $124.00
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Human Epi Growth Medium Kit for HPrEpC: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 215K-500 Price: $135.00
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Human Epi Growth Supplement for HPrEpC: Added to Basal Medium to create Growth Medium Size: 5 ml (3 parts) CAT.#: 215-GS Price: $70.00
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Human EpiVita Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 140-500 Price: $96.00
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Extended Family Products

Product Size CAT.# Price Quantity
Monoclonal IL-1 Receptor Antagonist Antibody: Monoclonal IL-1 Receptor Antagonist Antibody Size: 100 ul CAT.#: CB19846 Price: $302.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $160.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $41.00
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Cytofect Epithelial Cell Transfection Kit (124 x 24-Wells): 124 x 24-Well Rxns Size: 1 Kit CAT.#: TF102K Price: $465.00
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Cytofect Epithelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF102KS Price: $62.00
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Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor Size: 100 ug CAT.#: RP1026-100 Price: $86.00
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Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor Size: 500 ug CAT.#: RP1026-500 Price: $194.00
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Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor Size: 1000 ug CAT.#: RP1026-1000 Price: $264.00
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Prostate Epithelal Cell RNA (HPrEpC RNA), Adult: Total RNA prepared from Human Prostate Epithelial Cells, adult Size: 10 ug CAT.#: 934-R10a Price: $465.00
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Prostate Epithelal Cell RNA (HPrEpC RNA), Adult: Total RNA prepared from Human Prostate Epithelial Cells, adult Size: 25 ug CAT.#: 934-R25a Price: $930.00
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Human IL-1 alpha ELISA Kit: Human Interleukin-1 alpha ELISA Kit Size: 96 wells CAT.#: CL0389 Price: $484.00
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Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha Size: 10 ug CAT.#: RP1173-10 Price: $194.00
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Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha Size: 100 ug CAT.#: RP1173-100 Price: $624.00
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Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha Size: 1000 ug CAT.#: RP1173-1000 Price: $5,327.00
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Human IL-1 beta ELISA Kit: Human Interleukin-1 beta ELISA Kit Size: 96 wells CAT.#: CL0392 Price: $587.00
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Human Prostate RNA: Total RNA prepared from human prostate tissue Size: 50 ug CAT.#: 1H52-50 Price: $158.00
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Human Prostate RNA: Total RNA prepared from human prostate tissue Size: 250 ug CAT.#: 1H52-250 Price: $595.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $63.00
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Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free Size: 100 ug CAT.#: RP1026AF-100 Price: $95.00
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Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free Size: 500 ug CAT.#: RP1026AF-500 Price: $213.00
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Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free Size: 1000 ug CAT.#: RP1026AF-1000 Price: $290.00
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Resources/Documents

Cell Apps Flyer Epithelial Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now

Publications

 2013
Holtzhausen, A., C. Golzio, T. How, Y.-H. Lee, W.P. Schiemann, N. Katsanis, and G.C. Blobe. 2013. Novel bone morphogenetic protein signaling through Smad2 and Smad3 to regulate cancer progression and development. The FASEB Journal. 28:1-20.