Instructions HMSC.
Instructions HMSC-AD
MSDS Cryopreserved Cells
Cell Apps Flyer Skeletal System Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Description
Human Mesenchymal Stem Cells (HMSC) can maintain multipotency and proliferate extensively in vitro, providing new avenues for cell-based therapy in the restoration of damaged or diseased tissue. Bone marrow is the major blood creating organ, but in addition to supporting hematopoietic growth and differentiation, marrow stromal cells can be induced to produce cells of other connective tissues, such as bone, cartilage, and fat, as well as cells from neuroectodermal (neurons) and endodermal (hepatocytes) lineages.
Human Mesenchymal Stem Cells are also found in adipose tissue, and similarly possess the ability to differentiate into several different cell lineages.
Human Mesenchymal Stem Cellscan maintain multipotency and proliferate extensively in vitro, providing new avenues for cell-based therapy in the restoration of damaged or diseased tissue. HMSC are an immune-privileged cell type with both anti-inflammatory and anti-fibrotic capabilities, which can either be cell-based or through the vehicle of HMSC-derived extracellular vesicles.
Human Mesenchymal Stem Cellsare also being explored in numerous clinical applications involving autoimmune, cardiovascular, neurodegenerative, bone and cartilage diseases.
HMSC are capable of forming bone, cartilage, muscle and fat. As such they can potentially form tissues utilizing 3D printed scaffolds.
Cell Applications is proud to offer three varieties of HMSCs, each with their own distinctive properties: bone marrow stromal-derived (HMSC-BM), adipose stromal-derived (HMSC-AD), and dedifferentiated/defatted adipocytes (HMSC-DFAT)
Read about HMSC in "Mesenchymal Stem Cells - Global Strategic Business Report," ID: 2769226, September 2016, by Global Industry Analysts, Inc
HMSC from Cell Applications, Inc. have been utilized to investigate
Human Mesenchymal Stem Cellsare also being explored in numerous clinical applications involving autoimmune, cardiovascular, neurodegenerative, bone and cartilage diseases.
HMSC are capable of forming bone, cartilage, muscle and fat. As such they can potentially form tissues utilizing 3D printed scaffolds.
Cell Applications is proud to offer three varieties of HMSCs, each with their own distinctive properties: bone marrow stromal-derived (HMSC-BM), adipose stromal-derived (HMSC-AD), and dedifferentiated/defatted adipocytes (HMSC-DFAT)
Read about HMSC in "Mesenchymal Stem Cells - Global Strategic Business Report," ID: 2769226, September 2016, by Global Industry Analysts, Inc
HMSC from Cell Applications, Inc. have been utilized to investigate
- Effects of TGF-β1 on differentiation toward different lineages, and show that it inhibits adipogenic differentiation and induces either chondrogenic, osteogenic or smooth muscle cell differentiation, depending the specific culture conditions
- The differentiation potential of stem cells
- HLA expression profiles of bone marrow-derived and adipose-derived stem cells
- Heart valve-shaped tri-layered collagen-based constructs for stem cell seeding and differentiation to use in heart valve tissue engineering
(Click to Enlarge) Human Mesenchymal Stem Cells (HMSC) from bone marrow (A) differentiating into multiple lineages. Adipogenic - Oil Red O staining (B), Chondrogenic - Alcian Blue Staining (C) and Osteogenic - Alzarin Red S staining (D).
Details
Tissue | Normal healthy human bone marrow (HMSC) or adipose tissue (HMSC-AD) |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Character | Bone mineralization in Osteoblast Differentiation Med, Lipid accumulation in Adipocyte Diff Medium |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 HMSC frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen HMSC (492-05f, 492-05a, or 492Ad-05a), Growth Medium (419-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 10 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Mesenchymal Stem Cell Total Kit, fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 492K-05f | Price: $933.00 | |
| Cryopreserved Mesenchymal Stem Cells (HMSC), fetal: Frozen HMSC (5x10^5) | Size: 1 Cryovial | CAT.#: 492-05f | Price: $740.00 | |
| Proliferating Mesenchymal Stem Cells (HMSC), fetal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 493-25f | Price: $740.00 | |
| Proliferating Mesenchymal Stem Cells (HMSC), fetal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 493-75f | Price: $930.00 | |
| Proliferating Mesenchymal Stem Cells (HMSC), fetal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 493-96Wf | Price: $1,050.00 | |
| Cryopreserved Human Mesenchymal Stem Cell Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 492K-05a | Price: $933.00 | |
| Cryopreserved Mesenchymal Stem Cells (HMSC), adult: Frozen HMSC (5x10^5) | Size: 1 Cryovial | CAT.#: 492-05a | Price: $740.00 | |
| Proliferating Mesenchymal Stem Cells (HMSC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 493-25a | Price: $740.00 | |
| Proliferating Mesenchymal Stem Cells (HMSC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 493-75a | Price: $930.00 | |
| Proliferating Mesenchymal Stem Cells (HMSC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 493-96Wa | Price: $1,050.00 | |
| Cryopreserved Human Mesenchymal Stem Cell, Adipose-derived, Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 492AdK-05a | Price: $933.00 | |
| Cryopreserved Mesenchymal Stem Cells, Adipose-derived (HMSC-Ad), adult: Frozen HMSC-AD (5x10^5) | Size: 1 Cryovial | CAT.#: 492Ad-05a | Price: $740.00 | |
| Proliferating Mesenchymal Stem Cells, Adipose-derived (HMSC-Ad), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 493Ad-25a | Price: $740.00 | |
| Proliferating Mesenchymal Stem Cells, Adipose-derived (HMSC-Ad), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 493Ad-75a | Price: $930.00 | |
| Proliferating Mesenchymal Stem Cells, Adipose-derived (HMSC-Ad), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 493Ad-96Wa | Price: $1,050.00 | |
| Cryopreserved Human Mesenchymal Stem Cell, Dedifferetiated Fat, Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 492DFATK-05a | Price: $933.00 | |
| Cryopreserved Mesenchymal Stem Cells, Dedifferentated Fat (HMSC-DFAT), adult: Frozen HMSC-DFAT (5x10^5) | Size: 1 Cryovial | CAT.#: 492DFAT-05a | Price: $740.00 | |
| Proliferating Mesenchymal Stem Cells, Dedifferentiated Fat (HMSC-DFAT), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 493DFAT-25a | Price: $740.00 | |
| Proliferating Mesenchymal Stem Cells, Dedifferentiated Fat (HMSC-DFAT), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 493DFAT-75a | Price: $930.00 | |
| Proliferating Mesenchymal Stem Cells, Dedifferentiated Fat (HMSC-DFAT), adult: Actively growing, dividing cells in medium | Size: 493DFAT-96Wa | CAT.#: 493DFAT-96Wa | Price: $1,050.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| HMSC Growth Medium: All-in-one ready-to-use HMSC Growth Medium | Size: 500 ml | CAT.#: 419-500 | Price: $140.00 | |
| HMSC Growth Medium Kit: Basal HSMC medium & HSMC growth supplement sold together packaged separately | Size: yields 500 ml | CAT.#: 419K-500 | Price: $150.00 | |
| HMSC Basal Medium: Basal HMSC medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 418-500 | Price: $81.00 | |
| HMSC Growth Supplement: Added to HMSC Basal Medium to create HMSC Growth Medium | Size: 40 ml | CAT.#: 419-GS | Price: $88.00 | |
| HMSC Serum-Free Growth Medium: Serum-Free HSMC Growth Medium | Size: 500 ml | CAT.#: 419sf-500 | Price: $210.00 | |
| HMSC Serum-Free Growth Medium Kit: Serum-Free HMSC Growth Medium Kit | Size: Yields 500 ml | CAT.#: 419sfK-500 | Price: $216.00 | |
| HMSC Serum-Free Basal Medium : Serum-Free Basal HMSC Medium | Size: 500 ml | CAT.#: 418sf-500 | Price: $117.00 | |
| HMSC Serum-Free Growth Supplement: Serum-Free HMSC Growth Supplement | Size: 5 ml | CAT.#: 419sf-GS | Price: $140.00 | |
| HMSC Xeno-Free Basal Medium: Xeno-Free Basal HMSC Medium | Size: 500 ml | CAT.#: 418xf-500 | Price: $215.00 | |
| HMSC Growth Medium wo Antibiotics: HMSC Growth Medium without Antibiotics | Size: 500 ml | CAT.#: 419A-500 | Price: $140.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Anti-SCF: Rabbit Stem Cell Factor Antibody | Size: 100 ul | CAT.#: CG1536 | Price: $325.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Mesenchymal Stem Cell RNA (HMSC RNA), Adult: Total RNA prepared from Human Human Marrow Stromal Cells, adult | Size: 10 ug | CAT.#: 492-R10a | Price: $465.00 | |
| Mesenchymal Stem Cell RNA (HMSC RNA), Adult: Total RNA prepared from Human Human Marrow Stromal Cells, adult | Size: 25 ug | CAT.#: 492-R25a | Price: $930.00 | |
| Mesenchymal Stem Cell RNA (HMSC RNA), Fetal: Total RNA prepared from Human Human Marrow Stromal Cells, fetal | Size: 10 ug | CAT.#: 492-R10f | Price: $465.00 | |
| Mesenchymal Stem Cell RNA (HMSC RNA), Fetal: Total RNA prepared from Human Human Marrow Stromal Cells, fetal | Size: 25 ug | CAT.#: 492-R25f | Price: $930.00 | |
| Human Macrophage Colony Stimulating Factor (M-CSF): Human Macrophage-Colony Stimulating Factor | Size: 10 ug | CAT.#: RP1003-10 | Price: $194.00 | |
| Human Macrophage Colony Stimulating Factor (M-CSF): Human Macrophage-Colony Stimulating Factor | Size: 100 ug | CAT.#: RP1003-100 | Price: $624.00 | |
| Human Macrophage Colony Stimulating Factor (M-CSF): Human Macrophage-Colony Stimulating Factor | Size: 1000 ug | CAT.#: RP1003-1000 | Price: $5,327.00 | |
| Human M-CSF ELISA Kit: Human Macrophage-Colony Stimulating Factor ELISA Kit | Size: 96 Wells | CAT.#: CL0444 | Price: $511.00 | |
| Human Interleukin-7 (IL-7): Human Interleukin-7 | Size: 10 ug | CAT.#: RP1011-10 | Price: $194.00 | |
| Human Interleukin-7 (IL-7): Human Interleukin-7 | Size: 100 ug | CAT.#: RP1011-100 | Price: $624.00 | |
| Human Interleukin-7 (IL-7): Human Interleukin-7 | Size: 1000 ug | CAT.#: RP1011-1000 | Price: $5,327.00 | |
| Human IL-7 ELISA Kit: Human Interleukin-7 ELISA Kit | Size: 96 Wells | CAT.#: CL0779 | Price: $587.00 | |
| Human Macrophage Inflammatory Protein-3 Beta (MIP-3 beta/CCL19): Human Macrophage Inflammatory Protein-3 beta | Size: 20 ug | CAT.#: RP1135-20 | Price: $194.00 | |
| Human Macrophage Inflammatory Protein-3 Beta (MIP-3 beta/CCL19): Human Macrophage Inflammatory Protein-3 beta | Size: 100 ug | CAT.#: RP1135-100 | Price: $484.00 | |
| Human Macrophage Inflammatory Protein-3 Beta (MIP-3 beta/CCL19): Human Macrophage Inflammatory Protein-3 beta | Size: 1000 ug | CAT.#: RP1135-1000 | Price: $3,175.00 | |
| Human Stem Cell Factor (SCF): Human Stem Cell Factor | Size: 10 ug | CAT.#: RP1004-10 | Price: $194.00 | |
| Human Stem Cell Factor (SCF): Human Stem Cell Factor | Size: 100 ug | CAT.#: RP1004-100 | Price: $484.00 | |
| Human Stem Cell Factor (SCF): Human Stem Cell Factor | Size: 1000 ug | CAT.#: RP1004-1000 | Price: $3,175.00 | |
| Human Stromal Cell-Derived Factor-1 Alpha (SDF-1 alpha/CXCL12): Human Stromal Cell-Derived Factor-1-alpha | Size: 10 ug | CAT.#: RP1020-10 | Price: $194.00 | |
| Human Stromal Cell-Derived Factor-1 Alpha (SDF-1 alpha/CXCL12): Human Stromal Cell-Derived Factor-1-alpha | Size: 100 ug | CAT.#: RP1020-100 | Price: $484.00 | |
| Human Stromal Cell-Derived Factor-1 Alpha (SDF-1 alpha/CXCL12): Human Stromal Cell-Derived Factor-1-alpha | Size: 1000 ug | CAT.#: RP1020-1000 | Price: $3,175.00 | |
| Human Stromal Cell-Derived Factor-1 Beta (SDF-1 beta/CXCL12): Human Stromal Cell-Derived Factor-1-beta | Size: 10 ug | CAT.#: RP1159-10 | Price: $194.00 | |
| Human Stromal Cell-Derived Factor-1 Beta (SDF-1 beta/CXCL12): Human Stromal Cell-Derived Factor-1-beta | Size: 100 ug | CAT.#: RP1159-100 | Price: $484.00 | |
| Human Stromal Cell-Derived Factor-1 Beta (SDF-1 beta/CXCL12): Human Stromal Cell-Derived Factor-1-beta | Size: 1000 ug | CAT.#: RP1159-1000 | Price: $3,175.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| HPAd Differentiation Medium: Add to preadipocytes and mesenchymal stem cells to differentiate into adipocytes | Size: 250 ml | CAT.#: 811D-250 | Price: $109.00 | |
| HOb Differentiation Medium: Promotes cells to change from one type to another, more specialized | Size: 250 ml | CAT.#: 417D-250 | Price: $138.00 | |
| Human M-CSF, Animal-Free: Human Macrophage-Colony Stimulating Factor, Animal-Free | Size: 1000 ug | CAT.#: RP1003AF-1000 | Price: $5,860.00 | |
| Human M-CSF, Animal-Free: Human Macrophage-Colony Stimulating Factor, Animal-Free | Size: 100 ug | CAT.#: RP1003AF-100 | Price: $687.00 | |
| Human M-CSF, Animal-Free: Human Macrophage-Colony Stimulating Factor, Animal-Free | Size: 10 ug | CAT.#: RP1003AF-10 | Price: $213.00 | |
| Human Stem Cell Factor (SCF), Animal-Free: Human Stem Cell Factor, Animal-Free | Size: 1000 ug | CAT.#: RP1004AF-1000 | Price: $3,492.00 | |
| Human Stem Cell Factor (SCF), Animal-Free: Human Stem Cell Factor, Animal-Free | Size: 100 ug | CAT.#: RP1004AF-100 | Price: $533.00 | |
| Human Stem Cell Factor (SCF), Animal-Free: Human Stem Cell Factor, Animal-Free | Size: 10 ug | CAT.#: RP1004AF-10 | Price: $213.00 |
Resources/Documents
Publications
2016
Bellayr, I., R. Marklein, J. Lo Surdo, S. Bauer and R. Puri. 2016. Identification of Predictive Gene Markers for Multipotent Stromal Cell Proliferation. Stem Cells & Dev, 25:861-873.
2017
Tsao, Y., Y. Shih, Y. Liu, Y. Liu and O. Lee. 2017. Knockdown of SLC41A1 magnesium transporter promotes mineralization and attenuates magnesium inhibition during osteogenesis of mesenchymal stromal cells. Stem Cell Res & Ther, 8:39.
2016
Bellayr, I., R. Marklein, J. Lo Surdo, S. Bauer and R. Puri. 2016. Identification of Predictive Gene Markers for Multipotent Stromal Cell Proliferation. Stem Cells & Dev, 25:861-873.
Tan, C., H. Shichinohe, Z. Wang, S. Hamauchi, T. Abumiya, N. Nakayama, K. Kazumata, T. Ito, K. Kudo, S. Takamoto and K. Houkin. 2016. Feasibility and efficiency of human bone marrow stromal cell culture with allogeneic platelet lysate-supplementation for cell therapy against stroke.Stem Cells Intl, 6104780.
2015
Teng, N., Y. Liu, H. Wu, Y. Liu, J. Ho, and O. Lee. 2015. Promotion of Mesenchymal-to-Epithelial Transition by Rac1 Inhibition with Small Molecules Accelerates Hepatic Differentiation of Mesenchymal Stromal Cells. Tissue Engineering Part A. 21:1444-1454.
2014
Chang, H., D. Docheva, U. Knothe and M. Tate. 2014. Arthritic Periosteal Tissue From Joint Replacement Surgery: A Novel, Autologous Source of Stem Cells. Stem Cells & Regen Med, 3:308-317.
Gadkari, R., L. Zhao, T. Teklemariam, and B. Hantash. 2014. Human embryonic stem cell derived-mesenchymal stem cells: an alternative mesenchymal stem cell source for regenerative medicine therapy. Regenerative Medicine, 9:453-465.
Hantash, B. 2014. Differentiation of mesenchymal stem cells into fibroblasts, compositions comprising mesenchymal stem cell-derived fibroblasts, and methods of using the same. Patent US8822215 B2.
Matsubara, Y., T. Zama, Y. Ikeda, Y. Uruga, T. Suda and S. Matsuoka. 2014. Method for producing megakaryocytes, platelets and/or thrombopoietin using mesenchymal cells. Patent Application US 20160177265 A1.
Purandare, B., T. Teklemariam, L. Zhao, and B. Hantash. 2014. Temporal HLA profiling and immunomodulatory effects of human adult bone marrow-and adipose-derived mesenchymal stem cells. Regenerative Medicine 9: 67-79.
Teklemariam, T., B. Purandare, L. Zhao, and B. Hantash. 2014. Inhibition of DNA methylation enhances HLA-G expression in human mesenchymal stem cells. BBRC 452:753-759.
2013
Purandare, B., T. Teklemariam, L. Zhao, and B.M. Hantash. 2013. Temporal HLA profiling and immunomodulatory effects of human adult bone marrow- and adipose-derived mesenchymal stem cells. Regenerative Medicine. 9:67-79.
2012
Kato, Y., I. Hirata, and M. Kanawa. 2012. Animal Cell Culture Kit, Method for Culturing Animal Cells, Method for Selective Culture of Animal Cells and Cell Differentiation Method. Patent Application US 20140030804 A1.
2011
Hirota, Y., and M. Hakoda. 2011. Relationship between dielectric characteristic by DEP levitation and differentiation activity for stem cells. Key Engineering Materials. 459:84-91.
Park, J., H. Yoon, B. Yoo, Y. Kim, and Y. Shin. 2011. Method for the preparation of dermal papilla tissue employing mesenchymal stem cells. Patent US 8017390 B2.
Tedder, M.E., A. Simionescu, J. Chen, J. Liao, and D.T. Simionescu. 2011. Assembly and testing of stem cell-seeded layered collagen constructs for heart valve tissue engineering. Tissue engineering. Part A. 17:25-36.
Zhao, L., and B.M. Hantash. 2011. TGF-b1 Regulates Differentiation of Bone Marrow Mesenchymal Stem Cells. Vitamins and hormones 87:127-141.
2010
Tedder, M.E.B. 2010. Characterization and Evaluation of a Novel Tissue Engineered Aortic Heart Valve Construct. Clemson University, PhD dissertation.
Tedder, M., A. Simionescu, J. Chen, J. Liao, and D. Simionescu. 2010. Assembly and Testing of Stem Cell-Seeded Layered Collagen Constructs for Heart Valve Tissue Engineering. Tissue Engineering Part A., 17:25-36.
2006
Baron, U., I. Turbachova, A. Hellwag, F. Eckardt, K. Berlin, U. Hoffmüller, P. Gardina, and S. Olek. 2006. DNA Methylation Analysis as a Tool for Cell Typing. Epigenetics 1:55-60.
Le Visage, C., S.W. Kim, K. Tateno, A.N. Sieber, J.P. Kostuik, and K.W. Leong. 2006. Interaction of human mesenchymal stem cells with disc cells: changes in extracellular matrix biosynthesis. Spine. 31:2036-2042.