MSDS Cryopreserved Cells
Instructions HBlEpC
Cell Apps Flyer Epithelial Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Human bladder epithelial cells (HBlEpC) are a powerful tool in studying bladder epithelial development and function, mechanisms of cancerogenesis, especially those related to urothelial carcinoma of the bladder, as well as the processes by which epithelial cells achieve and maintain their specialized plasma membrane domains, and how these functions are disrupted in disease.
HBlEpC from Cell Applications, Inc. have been used as a normal control in a study measuring expression of CD44v6, a potential novel biomarker for bladder cancer.
Characterization: the cells have a characteristic morphology consistent with an epithelial origin and are positive for epithelial cell marker cytokeratin 18.
(Click to Enlarge) Human Bladder Epithelial Cells: HBlEpC (L). HBlEpC immunofluorescently stained with anti-CK18 antibody (R).
Details
Tissue | Normal healthy human bladder |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 HBlEpC (2nd passage) in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen HBlEpC (938-05a), Grwth Med (217-500), Subculture Rgnt Kit (090K) |
Proliferating | Shipped in Tsfr Med, psg 3, flasks or plates |
Doublings | At least 5 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Bladder Epithelial Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 938K-05a | Price: $944.00 | |
| Cryopreserved Bladder Epithelial Cells (HBlEpC), adult: Frozen HBlEpC (5x10^5) | Size: 1 Cryovial | CAT.#: 938-05a | Price: $745.00 | |
| Proliferating Bladder Epithelial Cells (HBlEpC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 939-25a | Price: $745.00 | |
| Proliferating Bladder Epithelial Cells (HBlEpC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 939-75a | Price: $935.00 | |
| Proliferating Bladder Epithelial Cells (HBlEpC), adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: 939-24Wa | Price: $935.00 | |
| Proliferating Bladder Epithelial Cells (HBlEpC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 939-96Wa | Price: $1,055.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| HBlEpC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 217-500 | Price: $146.00 | |
| HBlEpC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: 217K-500 | Price: $159.00 | |
| HBlEpC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 5 ml (3 parts) | CAT.#: 217-GS | Price: $85.00 | |
| Human Epi Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 214-500 | Price: $84.00 | |
| Human EpiVita Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 140-500 | Price: $96.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Polyclonal Epidermal Growth Factor Receptor Antibody: Polyclonal Epidermal Growth Factor Receptor Antibody | Size: 100 ul | CAT.#: CB7682 | Price: $302.00 | |
| Monoclonal IL-1 Receptor Antagonist Antibody: Monoclonal IL-1 Receptor Antagonist Antibody | Size: 100 ul | CAT.#: CB19846 | Price: $302.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cytofect Epithelial Cell Transfection Kit (124 x 24-Wells): 124 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF102K | Price: $465.00 | |
| Cytofect Epithelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF102KS | Price: $62.00 | |
| Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor | Size: 100 ug | CAT.#: RP1026-100 | Price: $86.00 | |
| Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor | Size: 500 ug | CAT.#: RP1026-500 | Price: $194.00 | |
| Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor | Size: 1000 ug | CAT.#: RP1026-1000 | Price: $264.00 | |
| Human IL-1 alpha ELISA Kit: Human Interleukin-1 alpha ELISA Kit | Size: 96 wells | CAT.#: CL0389 | Price: $484.00 | |
| Human IL-1 beta ELISA Kit: Human Interleukin-1 beta ELISA Kit | Size: 96 wells | CAT.#: CL0392 | Price: $587.00 | |
| Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | Size: 10 ug | CAT.#: RP1173-10 | Price: $194.00 | |
| Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | Size: 100 ug | CAT.#: RP1173-100 | Price: $624.00 | |
| Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | Size: 1000 ug | CAT.#: RP1173-1000 | Price: $5,327.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free | Size: 100 ug | CAT.#: RP1026AF-100 | Price: $95.00 | |
| Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free | Size: 500 ug | CAT.#: RP1026AF-500 | Price: $213.00 | |
| Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free | Size: 1000 ug | CAT.#: RP1026AF-1000 | Price: $290.00 |
Resources/Documents
Publications
2015
Song, M., J. Lim, H. Yu, J. Park, J. Chun, J. Jeong, J. Heo, H. Kang, Y. Kim, Y. Cho, S. Kim, W. Oh, S. Choi, S. Jang, S. Park, D. Shin, and M. Choo. 2015. Mesenchymal stem cell therapy alleviates interstitial cystitis by activating Wnt signaling pathway. Stem Cell and Development, 6 March.
2014
Hattori, S., K. Kojima, K. Minoshima, M. Yamaha, M. Horie, T. Sawamura, A. Kikuchi, and T. Deguchi. 2014. Detection of Bladder Cancer by Measuring CD44v6 Expression in Urine With Real-time Quantitative Reverse Transcription Polymerase Chain Reaction. Urology, 83:1443.e9-1443.e15.