MSDS Cryopreserved Cells
Instructions BPASMC
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Airway Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Bovine Pulmonary Artery Smooth Muscle Cells (BPASMC) from Cell Applications, Inc. have been used in studies relevant to
- Pulmonary hypertension characterized by hyperproliferation of pulmonary artery smooth muscle cells, the process shown to be mediated by hypoxia-induced expression of Bcl-xl
- Additionally, they were used to elucidate the PKC-α dependent mechanisms of proMMP-2 activation and other signaling events in smooth muscle cells under pro-inflammatory conditions related to cell growth
Characterization: positive for smooth muscle cell specific alpha-actin expression
(Click to Enlarge) Bovine Pulmonary Artery Smooth Muscle Cells, BPASMC (L) immunolabeled for smooth muscle-specific alpha-actin using ABC method (R).
Details
Tissue | Normal healthy bovine pulmonary artery |
QC | No bacteria, yeast, fungi, mycoplasma |
Character | Smooth muscle specific α-actin positive |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 BPASMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen BPASMC (B352-05), Growth Medium (B311-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | Can be cultured at least 16 doublings |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Bovine Pulmonary Artery Smooth Muscle Cells Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: B352K-05 | Price: $889.00 | |
| Cryopreserved Bovine Pulmonary Artery Smooth Muscle Cells (BPASMC): Frozen BPASMC (5x10^5) | Size: 1 Cryovial | CAT.#: B352-05 | Price: $715.00 | |
| Proliferating Bovine Pulmonary Artery Smooth Muscle Cells (BPASMC): Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: B353-25 | Price: $715.00 | |
| Proliferating Bovine Pulmonary Artery Smooth Muscle Cells (BPASMC): Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: B353-75 | Price: $905.00 | |
| Proliferating Bovine Pulmonary Artery Smooth Muscle Cells (BPASMC): Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: B353-24W | Price: $905.00 | |
| Proliferating Bovine Pulmonary Artery Smooth Muscle Cells (BPASMC): Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: B353-96W | Price: $1,025.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Bovine SMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: B310-500 | Price: $74.00 | |
| Bovine SMC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: B311-500 | Price: $121.00 | |
| Bovine SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: B311K-500 | Price: $130.00 | |
| Bovine SMC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml (2 parts) | CAT.#: B311-GS | Price: $74.00 | |
| Bovine SMC Induction Medium: Provides signals that change cell behavior | Size: 250 ml | CAT.#: B311I-250 | Price: $71.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Smooth Muscle Cell Transfection Kit (125 x 24-Wells): 125 x 24-Well | Size: 1 Kit | CAT.#: TF350K | Price: $328.00 | |
| Cytofect Smooth Muscle Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF350KS | Price: $62.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Publications
2016
Chowdhury, A., J. Sarkar, P. Pramanik, T. Chakraborti, S. and S. Chakraborti. 2016. Cross talk between MMP2-Spm-Cer-S1P and ERK1/2 in proliferation of pulmonary artery smooth muscle cells under angiotensin II stimulation. Archives of Biochemistry and Biophysics, 603:91–101.
Chowdhury, A., S. Nandy, J. Sarkar, T. Chakraborti and S. Chakraborti. 2011. Inhibition of pro-/active MMP-2 by green tea catechins and prediction of their interaction by molecular docking studies. Molec Cell Biochem, DOI 10.1007/s11010-016-2903-y.
Sarkar, J., S. Nandy, A. Chowdhury, T. Chakraborti and S. Chakraborti. 2016. Inhibition of MMP-9 by green tea catechins and prediction of their interaction by molecular docking analysis. Biomed & Pharmacother, 84:340-347.
Sarkar, J., A. Chowdhury, T. Chakraborti and S. Chakraborti. 2016. Cross-talk between NADPH oxidase-PKCα-p38MAPK and NF-κB-MT1MMP in activating proMMP-2 by ET-1 in pulmonary artery smooth muscle cells. Molec & Cell Biochm, 415:13-28.
2015
Chakraborti, S., A. Chowdhury and T. Chakraborti. 2015. Cross-talk between p38MAPK and Giα in regulating cPLA2 activity by ET-1 in pulmonary smooth muscle cells. Mol Cell Biochem, 400: 107.
Chowdhury, A., J. Sarkar, T. Chakraborti and S. Chakraborti. 2015. Role of Spm–Cer-S1P signalling pathway in MMP-2 mediated U46619-induced proliferation of pulmonary artery smooth muscle cells: protective role of epigallocatechin-3-gallate. Cell Biochem & Function, 33:463-477.
Rahaman, S., K. Dey, T. Chakraborti and S. Chakraborti. 2015. Angiotensin II inhibits Na+/K+ATPase activity in pulmonary artery smooth muscle cells via glutathionylation and with the involvement of a 15.6 kDa inhibitor protein. Indian J Biochem & Biophys, 52:119-124.
2014
Chowdhury, A., S. Roy, T. Chakraborti, K. Dey and S. Chakraborti. 2014. Activation of proMMP-2 by U46619 occurs via involvement of p38MAPK-NFκB-MT1MMP signaling pathway in pulmonary artery smooth muscle cells. Molec & Cell Biochem, 385:53-68.
Rahaman, S., K. Dey, P. Das, S. Roy, T. Chakraborti and S. Chakraborti. 2014. Identification, purification and partial characterization of low molecular weight protein inhibitor of Na+/K+-ATPase from pulmonary artery smooth muscle cells. Molec & Cellular Biol, 393:309-317.
2013
Chowdhury, A., S. Roy, T. Chakraborti, K. Dey, and S. Chakraborti. 2013. Activation of proMMP-2 by U46619 occurs via involvement of p38MAPK-NFκB-MT1MMP signaling pathway in pulmonary artery smooth muscle cells. Molecular and cellular biochemistry:1-16.
Roy, S., T. Chakraborti, A. Chowdhury, and S. Chakraborti. 2013. Role of PKC-α in NF-κB–MT1-MMP–mediated activation of proMMP-2 by TNF-α in pulmonary artery smooth muscle cells. Journal of biochemistry. 153:289-302.
Shaikh, S., J. Sarkar, A. Pramanik, K. Karmakar, and S. Chakraborti. 2013. Effect of m-calpain in PKCα-mediated proliferation of pulmonary artery smooth muscle cells by low dose of ouabain. IJBB. 50:419-427.
2012
Dey, K., S. Roy, B. Ghosh, and S. Chakraborti. 2012. Role of protein kinase C in phospholemman mediated regulation of α2β1 isozyme of Na+K+ ATPase in caveolae of pulmonary artery smooth muscle cells. Biochimie. 94:991-1000.
2011
Roy, S., K. Samanta, T. Chakraborti, A. Chowdhury, and S. Chakraborti. 2011. Role of TGF-β1 and TNF-α in IL-1β mediated activation of proMMP-9 in pulmonary artery smooth muscle cells: Involvement of an aprotinin sensitive protease. Archives of biochemistry and biophysics. 513:61-69.
2009
Chakraborti, S., A. Chowdhury, P. Kar, P. Das, S. Shaikh, S. Roy, and T. Chakraborti. 2009. Role of protein kinase C in NADPH oxidase derived O2(*-)-mediated regulation of Kv–LVOCC axis under U46619 induced increase in [Ca2+] in pulmonary smooth muscle cells. Archives of biochemistry and biophysics. 487:123-130.
2007
Suzuki, Y.J., H. Nagase, C.M. Wong, S.V. Kumar, V. Jain, A.-M. Park, and R.M. Day. 2007. Regulation of Bcl-xL expression in lung vascular smooth muscle. American journal of respiratory cell and molecular biology. 36:678.