Product Sheet CC10028
Description
BACKGROUND Actin is a ubiquitous globular protein that is one of the most highly-conserved proteins known. It is found in two main states; G-actin is the globular monomeric form, whereas F-actin forms helical polymers. Both G- and F-actin are intrinsically flexible structures - a feature vital in actin\'s role as a dynamic filament network.
Actin has four major functions. Firstly, F-actin polymers form microfilaments - polar intracellular \'tracks\' for kinesin motor proteins, allowing the transport of vesicles, organelles and other cargo. Actin is a component of the cytoskeleton and links to alpha-actinin, E-cadherin and beta-catenin at adherens junctions. This gives mechanical support to cells and attaches them to each other and the extracellular matrix. In muscle cells, actin-rich thin filaments associate with myosin-rich thick filaments to form actomyosin myofibrils. Using energy from the hydrolysis of ATP, myofibrils undergo cyclic shortening through actin-myosin head interactions, which represents the mechanics of muscle contraction. Finally, actin has a role in cell motility through polymerization and depolymerization of fibrils.1
Beta actin, also known as cytoplasmic actin is a 42 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. It is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure.2 For this reason, measurement of beta-Actin is generally used as an internal control for experimental error.
Actin has four major functions. Firstly, F-actin polymers form microfilaments - polar intracellular \'tracks\' for kinesin motor proteins, allowing the transport of vesicles, organelles and other cargo. Actin is a component of the cytoskeleton and links to alpha-actinin, E-cadherin and beta-catenin at adherens junctions. This gives mechanical support to cells and attaches them to each other and the extracellular matrix. In muscle cells, actin-rich thin filaments associate with myosin-rich thick filaments to form actomyosin myofibrils. Using energy from the hydrolysis of ATP, myofibrils undergo cyclic shortening through actin-myosin head interactions, which represents the mechanics of muscle contraction. Finally, actin has a role in cell motility through polymerization and depolymerization of fibrils.1
Beta actin, also known as cytoplasmic actin is a 42 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. It is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure.2 For this reason, measurement of beta-Actin is generally used as an internal control for experimental error.
REFERENCES
1. Pollard, D.T. & Cooper, J.A.: Science 326:1208-1212, 2009
2. Mori, R. et al: Prostate 68:1555-60, 2008
2. Mori, R. et al: Prostate 68:1555-60, 2008
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Western Blot detection of beta actin proteins in Huvec cell and mouse liver tissue lysates using beta-actin Antibody.
Details
Cat.No.: | CC10028 |
Antigen: | Raised against a short peptide from human beta-Actin sequence. |
Isotype: | Mouse IgG1 |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 42 kDa |
Specificity/Sensitivity: | Detects endogenous beta-actin proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse Beta-Actin Antibody: Beta-Actin Mouse Monoclonal Antibody | Size: 100 ul | CAT.#: CC10028 | Price: $302.00 |