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Human Renal Proximal Tubular Epithelial Cells: HRPTEpC

Left: Human Renal Proximal Tubular Epithelial Cells, HRPTEpC
Right: HRPTEpC immunofluorescently stained with anti-CK19

The proximal tubule is the portion of the duct system of the nephron of the kidney which leads from Bowman\'s capsule to the loop of Henle.  It has a single epithelial cell layer. The luminal surface of the epithelial cells of the proximal tubule is covered with millions of microvilli that form a brush border and increase surface area.  HRPTEpCs have a pivotal role in kidney function including reabsorbtion of glucose and protein, and also play a part in the glucotoxicity associated with diabetes. 

Human Renal Proximal Tubular Epithelial Cells (HRPTEpC) provide a useful tool for studying various aspects of pathology and biology of  the human renal proximal tubular cells in vitroHRPTEpC from Cell Applications, Inc. have recently been used in a study of metabolic enzymes responding to energy depletion in renal cells (Jackson, 2014).

Characterization: the cells have a characteristic morphology consistent with an epithelial origin and are positive for epithelial cell marker cytokeratin 18.

Tissue:
Normal human kidney.  Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
Cryopreserved ampoule:
First or second passage, >500,000 cells in RenaEpi Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HRPTEpC (930-05), 500 ml RenaEpi Growth Medium (911-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in RenaEpi Cell Transfer Medium at third passage in either flasks or multiwell plates.
Population doublings:
Can be cultured at least 3 population doublings for adult HRPTEpC and 10 population doublings for neonatal HRPTEpC

Jackson, E.K., D.G. Gillespie, Z. Mi, D. Cheng, R. Bansal, K. Janesko-Feldman, and P.M. Kochanek. 2014. Role of 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase in the Renal 2',3'-cAMP-Adenosine Pathway. Am J Physiol Renal Physiol: 10.1152/ajprenal.00134.02014.

Products

Cryopreserved HRPTEpC, adult

Pricing
Description
Size
Cat. #
Price
Cryopreserved HRPTEpC, adult Contains an ampoule of cryopreserved HRPTEpC (930-05), 500 ml RenaEpi Growth Medium (911-500), and a Subculture Reagent Kit (090K). 1 Kit 930K-05a $770.00
Cryopreserved HRPTEpC, adult 1 Ampoule 930-05a $619.00

Cryopreserved HRPTEpC, fetal

Pricing
Description
Size
Cat. #
Price
Cryopreserved HRPTEpC, fetal Contains an ampoule of cryopreserved HRPTEpC (930-05), 500 ml RenaEpi Growth Medium (911-500), and a Subculture Reagent Kit (090K). 1 Kit 930K-05f $780.00
Cryopreserved HRPTEpC, fetal 1 Ampoule 930-05f $629.00

Cryopreserved HRPTEpC, neonatal

Pricing
Description
Size
Cat. #
Price
Cryopreserved HRPTEpC, neonatal Contains an ampoule of cryopreserved HRPTEpC (930-05), 500 ml RenaEpi Growth Medium (911-500), and a Subculture Reagent Kit (090K). 1 Kit 930K-05n $780.00
Cryopreserved HRPTEpC, neonatal 1 Ampoule 930-05n $629.00

Proliferating HRPTEpC, adult

Pricing
Description
Size
Cat. #
Price
Proliferating HRPTEpC, adult 6 well 931-6Wa $809.00
Proliferating HRPTEpC, adult 96 well 931-96Wa $929.00
Proliferating HRPTEpC, adult Cultured and shipped in RenaEpi Transfer Medium at third passage in either flasks or multiwell plates. T-25 Flask 931-25a $619.00
Proliferating HRPTEpC, adult T-75 Flask 931-75a $809.00

Proliferating HRPTEpC, fetal

Pricing
Description
Size
Cat. #
Price
Proliferating HRPTEpC, fetal Cultured and shipped in RenaEpi Transfer Medium at third passage in either flasks or multiwell plates. T-25 Flask 931-25f $629.00
Proliferating HRPTEpC, fetal T-75 Flask 931-75f $819.00
Proliferating HRPTEpC, fetal 6 well 931-6Wf $819.00
Proliferating HRPTEpC, fetal 96 well 931-96Wf $939.00

Proliferating HRPTEpC, neonatal

Pricing
Description
Size
Cat. #
Price
Proliferating HRPTEpC, neonatal Cultured and shipped in RenaEpi Transfer Medium at third passage in either flasks or multiwell plates. T-25 Flask 931-25n $629.00
Proliferating HRPTEpC, neonatal T-75 Flask 931-75n $819.00
Proliferating HRPTEpC, neonatal 6 well 931-6Wn $819.00
Proliferating HRPTEpC, neonatal 96 well 931-96Wn $939.00

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