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Bovine Pulmonary Artery Smooth Muscle Cells: BPASMC

Bovine Pulmonary Artery Smooth Muscle Cells, BPASMC (left). BPASMC immunolabeled for smooth muscle-specific alpha-actin (ABC method, right). 

BPASMC from Cell Applications, Inc. have been used in studies relevant to pulmonary hypertension characterized by hyperproliferation of pulmonary artery smooth muscle cells, the process shown to be mediated by hypoxia-induced expression of Bcl-xl (Suzuki, 2007).  Additionally, they were used to elucidate the PKC-α dependent mechanisms of proMMP-2 activation and other signaling events in smooth muscle cells under pro-inflammatory conditions related to cell growth (Chakraborti, 2009; Roy, 2011, 2013; Dey, 2012; Chowdhury, 2013; Shaikh, 2013).

Characterization: positive for smooth muscle cell specific alpha-actin expression.

Tissue:
Tunica intima and media of pulmonary artery.
Animal tissues were USDA inspected.  Each lot is tested negative for mycoplasma, bacteria, and fungi.
Cryopreserved ampoule:
2nd passage, >500,000 cells in Bovine Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved BPASMC (B352-05), 500 ml Bovine Smooth Muscle Cell Growth Medium (B311-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Bovine Smooth Muscle Cell Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings

Chakraborti, S., A. Chowdhury, P. Kar, P. Das, S. Shaikh, S. Roy, and T. Chakraborti. 2009. Role of protein kinase C in NADPH oxidase derived O2(*-)-mediated regulation of Kv–LVOCC axis under U46619 induced increase in [Ca2+] in pulmonary smooth muscle cells. Archives of biochemistry and biophysics. 487:123-130.

Chowdhury, A., S. Roy, T. Chakraborti, K. Dey, and S. Chakraborti. 2013. Activation of proMMP-2 by U46619 occurs via involvement of p38MAPK-NFκB-MT1MMP signaling pathway in pulmonary artery smooth muscle cells. Molecular and cellular biochemistry:1-16.

Dey, K., S. Roy, B. Ghosh, and S. Chakraborti. 2012. Role of protein kinase C in phospholemman mediated regulation of α2 β1 isozyme of Na+K+ ATPase in caveolae of pulmonary artery smooth muscle cells. Biochimie. 94:991-1000.

Roy, S., T. Chakraborti, A. Chowdhury, and S. Chakraborti. 2013. Role of PKC-α in NF-κB–MT1-MMP–mediated activation of proMMP-2 by TNF-α in pulmonary artery smooth muscle cells. Journal of biochemistry. 153:289-302.

Roy, S., K. Samanta, T. Chakraborti, A. Chowdhury, and S. Chakraborti. 2011. Role of TGF-β1 and TNF-α in IL-1β mediated activation of proMMP-9 in pulmonary artery smooth muscle cells: Involvement of an aprotinin sensitive protease. Archives of biochemistry and biophysics. 513:61-69.

Shaikh, S., J. Sarkar, A. Pramanik, K. Karmakar, and S. Chakraborti. 2013. Effect of m-calpain in PKCα-mediated proliferation of pulmonary artery smooth muscle cells by low dose of ouabain. IJBB. 50:419-427.

Suzuki, Y.J., H. Nagase, C.M. Wong, S.V. Kumar, V. Jain, A.-M. Park, and R.M. Day. 2007. Regulation of Bcl-xL expression in lung vascular smooth muscle. American journal of respiratory cell and molecular biology. 36:678.

Products

Bovine Smooth Muscle Cell Basal Medium

Pricing
Description
Size
Cat. #
Price
Bovine Smooth Muscle Cell Basal Medium Growth Medium without Growth Supplements 500 ml B310-500 $53.00

Bovine Smooth Muscle Cell Growth Medium

Pricing
Description
Size
Cat. #
Price
Bovine Smooth Muscle Cell Growth Medium All-in-one ready-to-use media 500 ml B311-500 $98.00

Bovine Smooth Muscle Cell Growth Medium Kit

Pricing
Description
Size
Cat. #
Price
Bovine Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml B311K-500 $105.00

Bovine Smooth Muscle Cell Growth Supplement

Pricing
Description
Size
Cat. #
Price
Bovine Smooth Muscle Cell Growth Supplement Added to Basal Medium to create Growth Medium 50 ml (2 parts) B311-GS $53.00

Bovine Smooth Muscle Cell Induction Medium

Pricing
Description
Size
Cat. #
Price
Bovine Smooth Muscle Cell Induction Medium Bovine Smooth Muscle Cell Induction Medium 250 ml B311I-250 $58.00

Cryopreserved BPASMC

Pricing
Description
Size
Cat. #
Price
Cryopreserved BPASMC Each kit contains an ampoule of >500,000 cryopreserved Bovine Pulmonary Artery Smooth Muscle Cells, 500 ml Bovine Smooth Muscle Cell Growth Medium, and a Subculture Reagent Kit (090K). 1 Kit B352K-05 $714.00
Cryopreserved BPASMC 1 Ampoule B352-05 $575.00

Proliferating BPASMC

Pricing
Description
Size
Cat. #
Price
Proliferating BPASMC 6 well B353-6W $765.00
Proliferating BPASMC 96 well B353-96W $885.00
Proliferating BPASMC Cultured and shipped at third passage in Bovine Smooth Muscle Cell Growth Medium. T-25 Flask B353-25 $575.00
Proliferating BPASMC T-75 Flask B353-75 $765.00

Subculture Reagent Kit

Pricing
Description
Size
Cat. #
Price
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51.00