BEnEpC from Cell Applications, Inc. provide an excellent model system to study many aspects of epithelial function and disease, especially those related to the endometrium and pregnancy-related signaling, such as seminal plasma induced prostaglandins F2α, (PGF2α), E2 (PGE2) and interleukin-6 release by endometrial cells (Norrby, 2012; Madej, 2012; Malgorzata, 2013), or the role of PLA in mediating oxytocin-stimulated production of prostaglandins (Tithof, 2007). BEnEpC have also been used to demonstrate the association between variants of gap junctions related genes and conception rate (Sugimoto, 2013), and to investigate cellular adherence and cytotoxicity of Histophilus somni (Hoshinoo, 2009).
Bovine endometrium epithelium. Animal tissues were USDA inspected. Each lot is tested negative for mycoplasma, bacteria, and fungi.
2nd passage, >500,000 cells in Bovine Endometrial Basal Media containing 10% FBS & 10% DMSO.
Ampoule of cryopreserved BEnEpC(B932-05), 500 ml Bovine Endometrial Cell Growth Media (B911-500), and a Subculture Reagent Kit (090K).
Shipped in Bovine Endometrial Cell Growth Media at 3rd passage in either flasks or multiwell dishes.
Can be cultured at least 10 doublings
Hoshinoo, K., K. Sasaki, A. Tanaka, L.B. Corbeil, and Y. Tagawa. 2009. Virulence attributes of Histophilus somni with a deletion mutation in the ibpA gene. Microbial Pathogenesis. 46:273-282.
Krishnaswamy, N., G. Danyod, P. Chapdelaine, and M.A. Fortier. 2009. Oxytocin Receptor Down-Regulation Is Not Necessary for Reducing Oxytocin-Induced Prostaglandin F2α Accumulation by Interferon-τ in a Bovine Endometrial Epithelial Cell Line. Endocrinology. 150:897-905.
Madej, M., M. Norrby, M.T. Madsen, A. Johannisson, C. Hansen, and A. Madej. 2012. The Effect of Boar Seminal Plasma on the Release of Prostaglandins and Interleukin-6 by Porcine Endometrial and Cervical Cells and Bovine Endometrial Cells. Reproduction in Domestic Animals. 47:113-124.
Malgorzata, M., H. Claus, J. Anders, and M. Andrzej. 2013. Heparin-binding proteins from boar seminal plasma affecting the release of prostaglandins and interleukin-6 by porcine endometrial and cervical cells and bovine endometrial cells. Natural Science. 5:21.
Norrby, M. 2010. Hormones and sex steroid receptors in the female pig. Vol. 2010. Swedish University of Agricultural Sciences, PhD dissertation.
Sugimoto, M., S. Sasaki, Y. Gotoh, Y. Nakamura, Y. Aoyagi, T. Kawahara, and Y. Sugimoto. 2013. Genetic variants related to gap junctions and hormone secretion influence conception rates in cows. Proceedings of the National Academy of Sciences. 10.1073/pnas.1309307110
Tithof, P.K., M.P. Roberts, W. Guan, M. Elgayyar, and J.D. Godkin. 2007. Distinct phospholipase A2 enzymes regulate prostaglandin E2 and F2alpha production by bovine endometrial epithelial cells. Reproductive Biology and Endocrinology. 5:16.