Instructions TF102K
Instructions TF102KS

Description

Transfection, the introduction of nucleic acid into mammalian cells, is a powerful technique used to study in vivo gene function and regulation. The Cytofect™ Epithelial Cell Transfection kit is a plasmid DNA delivery system specifically optimized to deliver DNA into a wide variety of epithelial cells. This kit contains Cytofect-1, Enhancer and Media for transfection and culturing cells. Viral Enhancer has the ability to complex with unmodified DNA via cationic Cytofect-1 and protect the transfected DNA from lysosomal degradation.  This results in high transfection efficiency and viability for these traditionally hard-to-transfect primary epithelial cells.
#Wells / Rxns
96 / 620
24 / 124
12 / 62
The Kit contains all necessary reagents, media and optimized protocols to carry out successful transfection of epithelial cells with high efficiency (40%-70%) and viability (70%-95%).
Products are for research use only. They are not intended for human, animal, or diagnostic applications. *Viral Enhancer (VE) is a replication-deficient, Adenovirus-derived formulation that does not contain replication competent virus. TR1 and VE are manufactured by Targeting Systems.

(Click to Enlarge) Transfection  of  HEK (Top) and HMEpC (Bottom) with a GFP-expression plasmid using the Cytofect™ Epithelial Cell Transfection Kit.

Details

Kit Contents:
Cyrofect-1
Viral Enhancer (VE)*
Antibiotics-Free Growth Medium
Transfection Medium
Transfection Medium with Serum
 
Designed to improve transfection efficiency and cell viability, Cytofect is a plasmid DNA delivery system containing Cytofect-2, Enhancer and Media for transfecting and culturing cells. The Enhancer is an endosomolytic peptide that complexes with DNA and Cytofect-2, then escorts the transfection complex to the nucleus. The results are high transfection efficiency and viability for traditionally hard-to-transfect cells.
Instructions TF102K

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Instructions TF102KS

Format: PDF

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Products

Product Size CAT.# Price Quantity
Cytofect Epithelial Cell Transfection Kit (124 x 24-Wells): 124 x 24-Well Rxns Size: 1 Kit CAT.#: TF102K Price: $465.00
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Cytofect Epithelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF102KS Price: $62.00
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Related Products

Product Size CAT.# Price Quantity
Cytofect-1 (CF1): Store at -20°C Size: 250 ul CAT.#: 051-250 Price: $175.00
-+
Cytofect-1 (CF1): Store at -20°C Size: 500 ul CAT.#: 051-500 Price: $283.00
-+
Cytofect-2 (CF2) : Store at 4°C Size: 250 ul CAT.#: 052-250 Price: $175.00
-+
Cytofect-2 (CF2) : Store at 4°C Size: 500 ul CAT.#: 052-500 Price: $283.00
-+
Peptide Enhancer (PE): Store at 4°C Size: 250 ul CAT.#: 053-250 Price: $141.00
-+
Peptide Enhancer (PE): Store at 4°C Size: 500 ul CAT.#: 053-500 Price: $236.00
-+
Viral Enhancer (VE): Store at -20°C Size: 250 ul CAT.#: 054-250 Price: $141.00
-+
Viral Enhancer (VE): Store at -20°C Size: 500 ul CAT.#: 054-500 Price: $236.00
-+
Cyyofect Transfection Medium: Cyyofect Transfection Medium Size: 500 ml CAT.#: TF56-500 Price: $54.00
-+

Resources/Documents

Publications

2016
Sahoo, S., D. Meijles, I. Ghouleh, M. Tandon, E. Cifuentes-Pagano, J. Sembrat, M. Rojas, E. Goncharova and P. Pagano. 2016. MEF2C-MYOCD and Leiomodin1 Suppression by miRNA-214 Promotes Smooth Muscle Cell Phenotype Switching in Pulmonary Arterial Hypertension. PLoS ONE, dx.doi.org/10.1371/journal.pone.0153780.
2015
Dokun, A., L. Chen, M. Okutsu, C. Farber, S. Hazarika, W. Jones, D. Craig, D. Marchuk, J. Lye, S. Shan, and B. Annex. 2015. ADAM12: A Genetic Modifier of Pre-clinical Peripheral Arterial Disease. Am J Physiol, DOI: 10.1152/ajpheart.00803.2014.
Zelko, I., and R. Folz. 2015. Regulation of Oxidative Stress in Pulmonary Artery Endothelium: Modulation of EC-SOD and NOX4 Expression Using HDAC Class I Inhibitors. Am J Respir Cell Mol Biol, DOI: 10.1165/rcmb.2014-0260OC.
2013
Chen, Z., T.C. Lai, Y.H. Jan, F.M. Lin, W.C. Wang, H. Xiao, Y.T. Wang, W. Sun, X. Cui, Y.S. Li, T. Fang, H. Zhao, C. Padmanabhan, R. Sun, D.L. Wang, H. Jin, G.Y. Chau, H.D. Huang, M. Hsiao, and J.Y.J. Shyy. 2013. Hypoxia-responsive miRNAs target argonaute 1 to promote angiogenesis. The Journal of Clinical Investigation. 123:1057-1067.
He, M., X. Liang, L. He, W. Wen, S. Zhao, L. Wen, Y. Liu, J.Y.J. Shyy, and Z. Yuan. 2013. Endothelial dysfunction in rheumatoid arthritis: the role of monocyte chemotactic protein-1-induced protein. Arteriosclerosis, Thrombosis, and Vascular Biology. 33:1384-1391.
Yan, Y., X. Li, K. Kover, M. Clements, and P. Ye. 2013. CREB participates in the IGFIstimulation cyclin D1 transcription. Developmental Neurobiology. 73:559-570.
2012
Jeon, Y.J., M.G. Jo, H.M. Yoo, S.H. Hong, J.M. Park, S.H. Ka, K.H. Oh, J.H. Seol, Y.K. Jung, and C.H. Chung. 2012. Chemosensitivity is controlled by p63 modification with ubiquitin-like protein ISG15. The Journal of Clinical Investigation. 122:2622-2636.