Rat Lung Fibroblasts

Rat Lung Fibroblasts (RLF)
Rat Lung Fibroblasts (RLF) are derived from the lung of adult Sprague Dawley rat. The cells are cryopreserved at second passage. RLF can be cultured and propagated 10 population doublings. It was found that TGF-beta and PDGF produced by stimulated macrophages are involved in the regulation of the expression of alpha1(1) collagen, of prolyl-4-hydroxylase and lysyloxidase in rat lung fibroblasts¹. Antisense calcyclin (S100A6) RNA expression was found to inhibit serum and mechanical strain induced fibroblasts proleferation². Activation of fibroblasts by TGF-beta(1) by interferon-gamma may have potential therapeutic benefits in bleomycin-induced pulmonary fibrosis³. Furthermore, sulfated proteoglycans play a key role in modulating pulmonary fibroblast extracellular matrix composition and are important mediators of elastolytic injury⁴, and addition of heparin-binding EGF-like growth factor to pulmonary fibroblasts increases FGF-2 mRNA and protein levels. Koslowski, R. et al, Exp. Toxicol Patholo. 2003 Nove; 55(4): 257-64. Breen EC, Tang K, J Cell Biochem, 2003 Mar 1; 88(4): 848-54 Venkatesan, N. et al Am J Physiol Lung Cell Mol Physiol. 2002 Oct’ 283(4): L806-14 Buczek-Thomas JA, et al, J Cell Physiol. 2002 Sep; 192(3): 294-303 Liu, J, et al, Am J Physiol Lung Cell Mol Physiolo. 2003 Nov; 285(5):L1106-15
		Characterization: Positive for fibroblast surface protein.
Healthy monitoring report of animals were performed by Harlan Sprague Dawley, Inc.

Product Name	Product Description	Size (vol)	Cat. No.
RLF Total Kit	Each kit contains an ampoule of cryopreserved RLF (R506-05), 500 ml of Rat Fibroblast Growth Medium (R116-500), a Subculture Reagent Kit (090K).	1 Kit	R506K-05
Cryopreserved RLF	(>500,000 cells in an ampoule) Cryopreserved at second passage in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	R506-05
Proliferating RLF In Flasks	Cultured and shipped in flasks at third passage in Rat Fibroblast Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	R507-25 R507-50 R507-75
Proliferating RLF In Multiwell Plates	Cultured and shipped in plates at third passage in Rat Fibroblast Growth Medium.	6 well 12 well 24 well 48 well 96 well	R507-6W R507-12W R507-24W R507-48W R507-96W
Rat Fibroblast Growth Medium	Optimized and ready for use	500 ml	R116-500
Rat Fibroblast Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	R116K-500
Rat Fibroblast Basal Medium	Add Growth Supplement before use	500 ml	R115-500
Rat Fibroblast Growth Supplement		50 ml	R116-GS
Subculture Reagent Kit	(100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution)	100 ml	090K