Rat Dermal Fibroblasts

Rat Dermal Fibroblasts

(RDF)

Rat Dermal Fibroblasts (RDF) are derived from the dermis of normal Sprague Dawley rats. They are cryopreserved at first passage and can be cultured and propagated at least 8 population doublings.

The arginase activity was significantly elevated in wound–derived fibroblasts from Lewis rats compared to normal dermal fibroblasts underlines the distinct regulation of l-arginine metabolism in wound-derived fibroblasts¹. RDF were modified genetically to act like Schwann’s cells to deliver nerve growth factor². The role of skin fibroblast elastase on the degeneration of elastic fiber caused by UV irradiation was tested on the rat hind leg³.

Witte, M.B. et al, J. Surg. Res. 105(1):35-42 (2002).
Patrick, C.W. et al, Ann. Plast. Surg. 47(6):660-5 (2001).
Tsukahara, K. et al, J. Invest. Dermatol. 117(3):671-7 (2001).

Characterization: Positive for fibroblast surface protein expression.

Healthy monitoring report of animals were performed by Harlan Sprague Dawley, Inc.

Product Name	Product Description	Size (vol)	Cat. No.
RDF Total Kit	Each kit contains an ampoule of cryopreserved RDF (R106-05), 500 ml of RDF Growth Medium (R116-500), and a Subculture Reagent Kit (090K).	1 Kit	R106K-05
Cryopreserved RDF	(>500,000 cells in an ampoule) Cryopreserved at first passage in RDF Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	R106-05
Proliferating RDF In Flasks	Cultured and shipped in flasks at second passage in RDF Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	R107-25 R107-50 R107-75
Proliferating RDF In Multiwell Plates	Cultured and shipped in plates at second passage in RDF Growth Medium.	6 well 12 well 24 well 48 well 96 well	R107-6W R107-12W R107-24W R107-48W R107-96W
RDF Growth Medium	Optimized and ready for use.	500 ml	R116-500
RDF Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	R116K-500
RDF Basal Medium	Add Growth Supplement before use	500 ml	R115-500
RDF Growth Supplement		50 ml	R116-GS
Subculture Reagent Kit	(100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution)	100 ml	090K