Rat Cardiac Fibroblasts

Rat Cardiac Fibroblasts (RCF)
Rat Cardiac Fibroblasts (RCF) are derived from the heart tissue of normal Sprague Dawley rats. They are cryopreserved at second passage and can be cultured and propagated at least 8 population doublings. Rat Cardiac Fibroblasts are known to play key roles in the development of cardiac disease and hypertrophy¹. The regulation of fibroblast growth after acute myocardial infarction was determined in RCF². Cardiac fibroblasts were examined as a potential source of pro-inflammatory cytokine IL-6 synthesis which had been implicated in the pathophysiology of heart disease³. RCF have also been used to study the autocrine/paracrine capability mediated by vasodilator such as adernomedullin⁴. RCF are used to investigate their role in local hormone metabolism⁵ . Nitric oxide (NO) production in RCF decreases cAMP accumulation largely by the cGMP-mediated activation of PDE2⁶ . IL-1 beta-induced inducible NO synthase expression can trigger NO-dependent apoptosis in adult RCF⁷. Mareinfeld, U. et al, Basic Res. Cardiolo. 96(2):184-191 (2001) Staufenberger, S. et al, J. Cell. Physiol. 187(3):326-335 (2001) Burger, A. et al, Am. J. Physiol. Circ. Physiol. 281(1):H14-H21 (2001) Tomoda, Y. et al, Cariosac Res. 49(4):721-730 (2001) van der Hides, S.M. et al, J. Endocrinol. 174(1):111-9 (2002). Gustafsson, A.B., Brunton, L.L. Am. J. Physiol. Cell Physiol. 283(2):C463-71 (2002). Tian, B. et al, Am. J. Physiol. Heart Circ. Physiol. 283(5):H1958-67 (2002).

Healthy monitoring report of animals were performed by Harlan Sprague Dawley, Inc.

Product Name	Product Description	Size	Cat. No.
RCF Total Kit N (neonatal)/ A (adult)	Each kit contains an ampoule of cryopreserved RCF (R306-05), 500 ml of Rat Fibroblast Growth Medium (R116-500), and a Subculture Reagent Kit.	1 Kit	R306K-05a/n
Cryopreserved RCF	(>500,000 cells in an ampoule) Cryopreserved at second passage in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	R306-05a/n
Proliferating RCF In Flasks	Cultured and shipped in flasks at third passage in Rat Fibroblast Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	R307-25a/n R307-50a/n R307-75a/n
Proliferating RCF In Multiwell Plates	Cultured and shipped in plates at third passage in Rat Fibroblast Growth Medium.	6 well 12 well 24 well 48 well 96 well	R307-6Wa/n R307-12Wa/n R307-24Wa/n R307-48Wa/n R307-96Wa/n
Rat Fibroblast Growth Medium	Optimized and ready for use	500 ml	R116-500
Rat Fibroblast Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	R116K-500
Rat Fibroblast Basal Medium	Add Growth Supplement before use	500 ml	R115-500
Rat Fibroblast Growth Supplement		50 ml	R116-GS
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K