Rat Aortic Smooth Muscle Cells

Rat Aortic Smooth Muscle Cells

(RAOSMC)

Rat Aortic Smooth Muscle Cells (RAOSMC) are derived from the tunica intima and tunica media of healthy, fibrous plaque-free rat aorta. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. Increased arterial smooth muscle cell mass are found in the intimal lesion of atherosclerosis¹. Rat aortic smooth muscle cells respond to various factors by cell proliferation² and hypertrophy³, which are prominent indicators of atherosclerosis in vascular diseases. RAOSMC are well suited for the study of large vessel smooth muscle cell growth and differentiation^4,5 and serve as in vitro model in correlation with live rat models.

Ross, R., Nature 362:801 (1993).
Heldin, C.H. et al, Mol. Cell. Endocrinol. 39:169 (1985).
Berk, B.C. et al, J. Biol. Chem. 265:17334 (1990).
Owen, G.K. et al, J. Cell Biol. 102:343 (1986).
Skalli, O. et al, J. Cell Biol. 103(6):2787 (1986).

Characterization: Positive for smooth muscle cell specific a - actin expression.

Healthy monitoring report of animals were performed by Harlan Sprague Dawley, Inc.

Product Name	Product Description	Size (vol)	Cat. No.
RAOSMC Total Kit	Each kit contains an ampoule of cryopreserved RAOSMC (R354-05), 500 ml of Rat SMC Growth Medium (R311-500), and a Subculture Reagent Kit (090K).	1 Kit	R354K-05
Cryopreserved RAOSMC	(>500,000 cells in an ampoule) Cryopreserved at second passage in Rat SMC Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	R354-05
Proliferating RAOSMC In Flasks	Cultured and shipped in flasks at third passage in Rat SMC Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	R355-25 R355-50 R355-75
Proliferating RAOSMC In Multiwell Plates	Cultured and shipped in plates at third passage in Rat SMC Growth Medium.	6 well 12 well 24 well 48 well 96 well	R355-6W R355-12W R355-24W R355-48W R355-96W
Rat SMC Growth Medium	Optimized and ready for use	500 ml	R311-500
Rat SMC Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	R311K-500
Rat SMC Basal Medium	Add Growth Supplement before use	500 ml	R310-500
Rat SMC Growth Supplement		50 ml	R311-GS
Rat SMC Differentiation Medium		250 ml	R311D-250
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
RAOSMC RNA	Total RNA prepared from RAOSMC	10 µg 25 µg	R354-R10 R354-R25