Rat Aortic Endothelial Cells

Rat Aortic Endothelial Cells

(RAOEC)

Rat Aortic Endothelial Cells (RAOEC) are isolated from aorta of healthy adult rats. They are cryopreserved at third passage and can be cultured and propagated to sixth passage and beyond without losing their morphologic and phenotypic characteristics. RAOEC is a relevant in vitro model for correlating with in vivo studies in rat as an animal model. RAOEC have been used as a model for the study of Atherosclerosis¹ and products synthesized by the endothelium^2,3. The interaction of aortic endothelium and smooth muscle cells regulate the level and distribution of glycosaminoglycans in the arterial wall, especially in the intima⁴. Co-culture of aortic endothelial cells and smooth muscle cells provide an ideal in vitro model for studying the interaction between these two cells types.

Ross, R., Nature 362:801 (1993)
Falcy, C., et. al., Collagen Rel. Res. 5:519 (1985)
McGuire, P.G., et.al., Microcirc.Endo.Lymph. 2:129 (1985)
Merrilees, M.J., et.al., Atherosclerosis 39:147 (1981)

DiI-Ac-LDL Uptake

Characterization: Positive for DiI-Ac-LDL uptake.

Healthy monitoring report of animals were performed by Harlan Sprague Dawley, Inc.

Product Name	Product Description	Size (vol)	Cat. No.
RAOEC Total Kit	Each kit contains an ampoule of cryopreserved RAOEC (R304-05), 500 ml of Rat Endothelial Cell Growth Medium (R211-500), 100 ml of Attachment Factor Solution (123-100), and a Subculture Reagent Kit.	1 Kit	R304K-05
Cryopreserved RAOEC	(>500,000 cells in an ampoule) Cryopreserved at third passage in Rat Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	R304-05
Proliferating RAOEC In Flasks	Cultured and shipped in flasks at fourth passage in Rat Endothelial Cell Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	R305-25 R305-50 R305-75
Proliferating RAOEC In Multiwell Plates	Cultured and shipped in flasks at fourth passage in Rat Endothelial Cell Growth Medium.	6 well 12 well 24 well 48 well 96 well	R305-6W R305-12W R305-24W R305-48W R305-96W
Rat Endothelial Cell Growth Medium	Optimized and ready for use	500 ml	R211-500
Rat Endothelial Cell Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	R211K-500
Rat Endothelial Cell Basal Medium	Add Growth Supplement before use	500 ml	R210-500
Rat Endothelial Cell Growth Supplement		15 ml	R211-GS
Attachment Factor Solution	Promotes attachment of endothelial cells.	100 ml	123-100
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
RAOEC RNA	Total RNA prepared from RAOEC	10 µg 25 µg	R304-R10 R304-R25