Porcine Smooth Muscle Cells

Porcine Coronary Artery Smooth Muscle Cells (PCASMC)

Porcine Pulmonary Artery Smooth Muscle Cells (PPASMC)

Porcine Aortic Smooth Muscle Cells (PAOSMC)

Porcine large artery smooth muscle cells are derived from tunica intima and tunica media of healthy, fibrous plaque-free porcine arteries. They are cryopreserved at first passage (PCASMC at second passage) and can be cultured and propagated at least 16 population doublings (PCASMC at least 14 doublings). Smooth muscle cells have been found in fatty streaks of early arteriosclerosis. Proliferation of the smooth muscle cells is considered a key event in the development of advanced lesions¹. Porcine smooth muscle cells serve as an alternative for the investigations of cardiac disease and arteriosclerosis^1-3. It can be used in co-culture investigation with species-matched porcine artery endothelial cells.

Ross, R.N., Nature 362:801 (1993).
Stary, H.C., Arteriosclerosis 3:471a (1983).
McGill, H.C. Jr., Arteriosclerosis 4:443 (1984).

Characterization: Smooth muscle cell specific a -actin expression.

Animal tissues were USDA inspected.

Product Name	Product Description	Size (vol)	Cat. No.
Porcine SMC Total Kit PCASMC PPASMC PAOSMC	Each kit contains an ampoule of cryopreserved Porcine Smooth Muscle Cells, 500 ml Porcine SMC Growth Medium, and a Subculture Reagent Kit (090K).	1 Kit	P350K-05 P352K-05 P354K-05
Cryopreserved Porcine SMC PCASMC PPASMC PAOSMC	(>500,000 cells in an ampoule) Cryopreserved at first passage (PCASMC at second passage) in Porcine SMC Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	P350-05 P352-05 P354-05
Proliferating Porcine SMC In Flasks PCASMC; PPASMC; PAOSMC PCASMC; PPASMC; PAOSMC PCASMC; PPASMC ;PCASMC	Cultured and shipped in flasks at second passage (PCASMC at third passage) in Porcine SMC Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	P351-25; P353-25; P355-25 P351-50; P353-50; P355-50 P351-75; P353-75; P355-75
Proliferating Porcine SMC In Multiwell Plates PCASMC; PPASMC; PAOSMC PCASMC; PPASMC; PAOSMC PCASMC; PPASMC; PCASMC PCASMC; PPASMC; PAOSMC PCASMC; PPASMC; PAOSMC	Cultured and shipped in plates at second passage (PCASMC at third passage) in Porcine SMC Growth Medium.	6 well 12 well 24 well 48 well 96 well	P351-6W; P353-6W; P355-6W P351-12W; P353-12W; P355-12W P351-24W; P353-24W; P355-24W P351-48W; P353-48W; P355-48W P351-96W; P353-96W; P355-96W
Porcine SMC Growth Medium	Optimized and ready for use	500 ml	P311-500
Porcine SMC Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	P311K-500
Porcine SMC Basal Medium	Add Growth Supplement before use	500 ml	P310-500
Porcine SMC Growth Supplement		50 ml	P311-GS
Porcine SMC Differentiation Medium		250 ml	P311D-250
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K