Porcine Endothelial Cells

Porcine Coronary Artery Endothelial Cells (PCAEC)

Porcine Pulmonary Artery Endothelial Cells (PPAEC)

Porcine Aortic Endothelial Cells (PAOEC)

Porcine large artery endothelial cells are derived from healthly, plaque free porcine. PAOEC and PPAEC are cryopreserved at first passage and can be cultured and propagated at least 16 population doublings. PCAEC is cryopreserved at second passage and can be cultured and propagated at least 12 population doublings. Arterial endothelial cells play critical roles in cardiac homeostasis. Insults to the endothelial cells have been linked to a variety of vascular diseases. Porcine artery endothelial cells have been used as a viable alternative for the study of artery endothelial function and metabolism, such as atherosclerosis¹, relaxing factor synthesis², antioxidant enzyme activities³, etc. Co-culture of the artery endothelial cells with species-matched smooth muscle cells provides an ideal model for studying the interaction between these two cell types.

Ross, R.N., Nature 362:801 (1993).
Boulanger, C.M. et al, J. fo Vasc. Res. 34(3):175 (1997).
Bishop, C.T. et al, In Vitro Cell Devel. Biol. 21:229 (1985).

Characterization: Positive for Dil-Ac-LDL uptake.

Animal tissues were USDA inspected.

Product Name	Product Description	Size (vol)	Cat. No.
Porcine EC Total Kit PCAEC PPAEC PAOEC	Each kit contains an ampoule of cryopreserved Porcine Endothelial Cells, 500 ml of Porcine Endothelial Cell Growth Medium (P211-500), and a Subculture Reagent Kit (090K).	1 Kit	P300K-05 P302K-05 P304K-05
Cryopreserved Porcine EC PCAEC PPAEC PAOEC	(>500,000 cells in an ampoule) Cryopreserved at first passage (PCAEC at second passage) in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	P300-05 P302-05 P304-05
Proliferating Porcine EC In Flasks PCAEC;PPAEC;PAOEC PCAEC;PPAEC;PAOEC PCAEC;PPAEC;PCAEC	Cultured and shipped in flasks at second passage (PCAEC at third passage) in Porcine Endothelial Cell Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	P301-25;P303-25;P305-25 P301-50;P303-50;P305-50 P301-75;P303-75;P305-75
Proliferating Porcine EC In Multiwell Plates PCAEC;PPAEC;PAOEC PCAEC;PPAEC;PAOEC PCAEC;PPAEC;PCAEC PCAEC;PPAEC;PAOEC PCAEC;PPAEC;PAOEC	Cultured and shipped in plates at second passage (PCAEC at third passage) in Porcine Endothelial Cell Growth Medium.	6 well 12 well 24 well 48 well 96 well	P301-6W;P303-6W;P305-6W P301-12W:P303-12W;P305-12W P301-24W;P303-24W;P305-24W P301-48W;P303-48W;P305-48W P301-96W;P303-96W;P305-96W
Porcine EC Growth Medium	Optimized and ready for use	500 ml	P211-500
Porcine EC Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	P211K-500
Porcine EC Basal Medium	Add Growth Supplement before use	500 ml	P210-500
Porcine EC Growth Supplement		50 ml	P211-GS
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
Porcine EC RNA PPAEC PAOEC	Total RNA prepared from Porcine Endothelial Cells	10 µg	P302-R10 P304-R10
Porcine EC RNA PPAEC PAOEC	Total RNA prepared from Porcine Endothelial Cells	25 µg	P302-R25 P304-R25