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Mouse
Neural Stem Cells (MNSC) are isolated from cerebral striatum and
mesencephalon of E14 fetal mouse brains. These cells were maintained in
an undifferentiated proliferative state by culturing them as free-floating
neurospheres in serum-free medium optimized with growth factors. MNSC are cryopreserved at second passage and can be cultured and propagated
for 1-2 passages prior to induction of phenotypic differentiation. These
stem cells are multipotent1,2 and
can be induced to differentiate into neurons, astrocytes and
oligodendrocytes. Neural stem cells are a source of donor tissue
for prospective neural cell therapy given the ability of neurosphere to retain
multi-lineage potential and proliferative ability in
vitro.
1. Reynolds, B.A. et al, J.
Neuroscience 12(11):4565 (1992).
2.
Reynolds, B.A. et al, Develop. Bio. 175:1 (1996). |
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MNSC
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Differentiated MNSC stained
for β-Tubulin
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