Re-Differentiated Human Chondrocytes

(RD-HC)

Chondrocytes in monolayer culture lose their phenotypic characteristics very quickly and became de-differentiated to fibroblast-like phenotype^1,2,3. Cartilage-specific Type II Collagen expression cannot be detected in these de-differentiated chondrocytes⁴. Now we provide Re-Differentiated chondrocytes by encapsulating chondrocytes in alginate bead suspension. These Re-Differentiated chondrocytes produce their phenotypic marker aggrecan and Type II Collagen again^14,5. Cells are shipped 10 days after encapsulation and Re-Differentiated choncdocytes are expected 3 weeks after encapsulation and should remain the phenotypic characteristics for at least one weeks after complete re-differentiation. These cells have been used for the repair of cartilage defects⁶.

Kato, Y. et al, J. Cell Physiol. 120:354 (1984)
Jennings, S. D. et al, Exp. Cell Res. 145:415 (1983)
Kuettner, K. E. et al, J. Cell Biol. 93:751 (1982)
Benya, P. D. et al, Cell 30:215 (1982)
Guo, J. et al, Connect. Tissue Res. 19:277 (1989)
Grande, D. A. et al, Biomed. Mat. Res. 34:211 (1997)

HC encapsulated in alginate beads

Characterization:

Exhibit spherical morphology when encapsulated in alginate beads.
Positive for aggrecan mRNA and Collagen Type II mRNA.

Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, and negative for mycoplasma, bacteria, yeast and fungi.

Product Name	Product Description	Size (vol)	Cat. No.
Proliferating RD-HC In Flasks adult	Encapsulated in alginate beads and shipped in flasks in Chondrocyte Differentiation Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	403RD-25a 403RD-50a 403RD-75a
Proliferating RD-HC In Flasks fetal	Encapsulated in alginate beads and shipped in flasks in Chondrocyte Differentiation Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	403RD-25f 403RD-50f 403RD-75f
Chondrocyte Growth Medium	Optimized and ready for use	500 ml	411-500
Chondrocyte Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	411K-500
Chondrocyte Basal Medium	Add Growth Supplement before use	500 ml	410-500
Chondrocyte Growth Supplement		50 ml	411-GS
Chondrocyte Differentiation Medium		250 ml	411D-250
Depolarization Solution		50 ml	073-50
Re-Differentiated Chondrocyte RNA	Total RNA prepared from RD-HC	10 µg	402RD-R10a (adult) 402RD-R10f (fetal)
Re-Differentiated Chondrocyte RNA	Total RNA prepared from RD-HC	25 µg	402RD-R25a (adult) 402RD-R25f (fetal)