HUVEC, Human Umbilical Vein Endothelial Cells

Human Umbilical Vein Endothelial Cells

(HUVEC)

Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from normal human umbilical vein. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings. HUVEC are responsive to cytokine stimulation in the expression of cell adhesion molecules. These cell systems are commonly used for physiological and pharmacological investigations, such as macromolecule transport¹, blood coagulation², and fibrinolysis³.

1. Weiss, T.L. et al, In Vitro Cell. Biol. 26:759 (1990).

2. Furie, B. et al, Cell 53:505 (1988).

3. Wojta, J. et al, Blood 81(12):3285 (1993).

HUVEC, Human Umbilical Vein Endothelial Cells, angiogenesis, VEGF

HUVEC

HUVEC, human umbilical vein endothelial cells tubule formation in matrigel

HUVEC Tubule Formation

in Matrigel

Characterization:

Positive for factor VIII-related antigen expression. Positive for DiI-Ac-LDL uptake.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

We have special lots that were verified to be responsive to VEGF. Please call to discuss how these lots can meet your needs. The activation of VEGF-R2 was validated by autophosphorylation at tyrosine 1175. VEGF-R2 is a high affinity receptor for VEGF, which stimulates proliferation, chemotaxis and sprouting of endothelial cells in vitro and angiogenesis in vivo via VEGF-R2 signaling pathway.

click here to go to VEGF page...

Product Name	Product Description	Size (vol)	Cat. No.
HUVEC Total Kit , neonatal	Each kit contains an ampoule of cryopreserved HUVEC (200-05n), 500 ml of Endothelial Cell Growth Medium (211-500), a Subculture Reagent Kit.	1 Kit	200K-05n
Cryopreserved HUVEC , neonatal Cryopreserved HUVEC , pooled, neonatal	(>500,000 cells in an ampoule) Cryopreserved in ampoules at the end of primary culture in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	200-05n 200p-05n
Proliferating HUVEC In Flasks neonatal	Cultured in flasks at first passage in Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	201-25n 201-50n 201-75n
Proliferating HUVEC , pooled In Flasks neonatal	Cultured in flasks at first passage in Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	201p-25n 201p-50n 201p-75n
Proliferating HUVEC , neonatal In Multiwell Plates	Cultured in plates at first passage in Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium.	6 well 12 well 24 well 48 well 96 well	201-6Wn 201-12Wn 201-24Wn 201-48Wn 201-96Wn
Proliferating HUVEC , pooled In Multiwell Plates neonatal	Cultured in plates at first passage in Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium.	6 well 12 well 24 well 48 well 96 well	201p-6Wn 201p-12Wn 201p-24Wn 201p-48Wn 201p-96Wn
Endothelial Cell Growth Medium	Optimized and ready for use	500 ml	211-500
Endothelial Cell Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	211K-500
Endothelial Cell Basal Medium	Add Growth Supplement before use	500 ml	210-500
Endothelial Cell Growth Supplement		15 ml	211-GS
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
HUVEC RNA , neonatal Stimulated HUVEC RNA , neonatal	Total RNA prepared from HUVEC Total RNA prepared from stimulated HUVEC	10 µg 25 µg 10 µg 25 µg	200-R10n 200-R25n 200S-R10n 200S-R25n