Human Umbilical Artery Smooth Muscle Cells

(HUASMC)

Human Umbilical Artery Smooth Muscle Cells (HUASMC) are isolated from normal human umbilical arteries. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. Umbilical artery smooth muscle cells have been used as an economical alternative for the study of morphological and structural changes in the development of atherosclerosis^1,2.

Ross, R.N., Nature 362:801 (1993).
McGill, H.C. Jr., Arteriosclerosis 4:443 (1984).

Characterization: Positive for smooth muscle cell specific a -actin expression.

Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, and negative for mycoplasma, bacteria, yeast and fungi.

Product Name	Product Description	Size (vol)	Cat. No.
HUASMC Total Kit, neonatal	Each kit contains an ampoule of cryopreserved HUASMC (252-05n), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit (090K).	1 Kit	252K-05n
Cryopreserved HUASMC, neonatal	(>500,000 cells in an ampoule) Cryopreserved in ampoules at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	252-05n
Proliferating HUASMC, neonatal In Flasks	Cultured and shipped in flasks at second passage in Smooth Muscle Cell Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	253-25n 253-50n 253-75n
Proliferating HUASMC, neonatal In Multiwell Plates	Cultured and shipped in plates at second passage in Smooth Muscle Cell Growth Medium.	6 well 12 well 24 well 48 well 96 well	253-6Wn 253-12Wn 253-24Wn 253-48Wn 253-96Wn
Smooth Muscle Cell Growth Medium	Optimized and ready for use.	500 ml	311-500
Smooth Muscle Cell Growth Medium Kit	Basal Medium & Growth Supplement packaged separately.	Yields 500 ml	311K-500
Smooth Muscle Cell Basal Medium	Add Growth Supplement before use.	500 ml	310-500
Smooth Muscle Cell Growth Supplement		30 ml	311-GS
Smooth Muscle Cell Differentiation Medium		250 ml	311D-250
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
HUASMC RNA , neonatal	Total RNA prepared from HUASMC.	10 µg 25 µg	252-R10n 252-R25n