Human Umbilical Artery Endothelial Cells
(HUAEC)
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Human Umbilical Artery Endothelial Cells
(HUAEC) are isolated from normal human umbilical arteries. They are cryopreserved at second passage and can be
cultured and propagated at least 15 population doublings. Arterial endothelial cells have been used as an
in vitro
model for the study of morphological and ultrastructural changes in the development of atherosclerosis1. The HUAEC system has also been used for the study of endothelial cell-dependent
lymphocyte activation .and chemoattractant activity2,3.
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Antonor,
A.S. et al, Atherosclerosis 59:1 (1986).
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Ashida,
E.R. et al, J. Clin. Inv. 67:1490 (1981).
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Farber,
H.W. et al, Amer. J. Physiol. 262 (4 parts):H1088 (1992).
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Characterization:
Positive for Factor VIII-related antigen expression. Positive for Factor VIII-related Dil-Ac-LDL uptake.
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Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, and negative for mycoplasma, bacteria, yeast and fungi.
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| Product Name
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Product Description
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Size (vol)
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Cat. No.
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| HUAEC Total Kit,
neonatal
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Each kit contains an ampoule of cryopreserved HUAEC (202-05n), 500 ml of HUAEC Growth Medium (212-500), and
a Subculture Reagent Kit.
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1 Kit
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202K-05n
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| Cryopreserved HUAEC,
neonatal
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(>500,000 cells in an ampoule)
Cryopreserved at first passage in Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO.
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1 Ampoule
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202-05n
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Proliferating HUAEC,
neonatal
In Flasks
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Cultured in flasks at second passage in HUAEC Growth Medium and shipped in Endothelial Cell Transfer Medium.
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T-25 Flask
2 T-25 Flasks
T-75 Flask or 3 T-25 Flasks
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203-25n
203-50n
203-75n
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Proliferating HUAEC,
neonatal
In Multiwell Plates
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Cultured in plates at second passage in HUAEC Growth Medium and shipped in Endothelial Cell Transfer Medium.
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6 well
12 well
24 well
48 well
96 well
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203-6Wn
203-12Wn
203-24Wn
203-48Wn
203-96Wn
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| HUAEC Growth Medium
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Optimized and ready for use
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500 ml
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212-500
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| HUAEC Growth Medium Kit
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Basal Medium & Growth Supplement packaged separately
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Yields 500 ml
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212K-500
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| HUAEC Basal Medium
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Add Growth Supplement before use
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500 ml
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210-500
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| HUAEC Growth Supplement
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30 ml
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212-GS
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| Subculture Reagent Kit
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100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution
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100 ml
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090K
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| HUAEC RNA,
neonatal
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Total RNA prepared from HUAEC
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10
µg
25
µg
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202-R10n
202-R25n
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