Human Subclavian Artery Smooth Muscle Cells

Human Subclavian Artery Smooth Muscle Cells (HScASMC)
Human subclavian artery smooth muscle cells (HScASMC ) are derived from the subclavian artery. They are cryopreserved at second passage. HScASMC can be cultured and propagated 12 population doublings. Morphology and growth characteristics of HScASMC from neonates were found to be different from those of adults¹. A rabbit model using the subclavian artery was developed to study the temporal development and characteristics of an intimal cellular response and functional changes following balloon angioplasty injury². Prolonged treatment with r-hirudin neither inhibits vascular smooth muscle cell proliferation in rabbits after angioplasty of the left subclavian artery nor reduces the size of neointimal lesion. This suggests r-hirudin treatment is not suitable as an antirestenotic intervention³. Subclavian-pulmonary artery anastomoses were created in Sprague-Dawley rats under different experimental conditions. It was found that the type of injury and the associated hemodynamic conditions could modify the elastic pulmonary response to injury⁴. Fujita H. et al, Exp Mol. Pathol. 1993 Feb; 58(1):25-39. Hadoke P, et al, Coron Artery Dis. 1995 May; 6(5):403-15. Hadoke PW, et al, Coron Artery Dis. 1996 Aug; 7(8):599-608. Tanaka Y, et al, J Clin Invest. 1996 Jul 15; 98(2):434-42.
		Characterization: Positive for smooth muscle cell specific a-actin expression.
Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, and negative for mycoplasma, bacteria, yeast and fungi.

Product Name	Product Description	Size (vol)	Cat. No.
HScASMC Total Kit, adult	Each kit contains an ampoule of cryopreserved HScASMC (3510-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit (090K).	1 Kit	3510K-05a
Cryopreserved HScASMC , adult	(>500,000 cells in an ampoule) Cryopreserved at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	3510-05a
Proliferating HScASMC , adult In Flasks	Cultured and shipped in flasks at third passage in Smooth Muscle Cell Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	3511-25a 3511-50a 3511-75a
Proliferating HScASMC , adult In Multiwell Plates	Cultured and shipped in plates at third passage in Smooth Muscle Cell Growth Medium.	6 well 12 well 24 well 48 well 96 well	3511-6Wa 3511-12Wa 3511-24Wa 3511-48Wa 3511-96Wa
Smooth Muscle Cell Growth Medium	Optimized and ready for use	500 ml	311-500
Smooth Muscle Cell Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	311K-500
Smooth Muscle Cell Basal Medium	Add Growth Supplement before use	500 ml	310-500
Smooth Muscle Cell Growth Supplement		30 ml	311-GS
Smooth Muscle Cell Differentiation Medium		250 ml	311D-250
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K