Human Preadipocytes

Human Preadipocytes

(HPAd)

Human Preadipocytes (HPAd) are derived from human adipose tissue. These fibroblast-like precursor cells are cryopreserved at the end of primary culture and can be propagated two passages prior to differentiating into Human Adipocytes (HAd). HPAd derived HAd were used to study insulin-stimulated glucose transport^1,2, growth hormone enhanced lypolysis³ and obese gene expression⁴. HPAd can be fully differentiated into mature HAd in CAI’s Adipocyte Differentiation Medium. Significant differences in lipolysis and leptin production⁴ are observed in HPAd and HAd from human omental and subcutaneous adipose tissue. We provide HPAd from subcutaneous adipose tissue at various sites and adipose depot on the heart. HPAd/HAd are a useful in vitro cellular model for investigating the causes and treatment of obesity, type II diabetes and cardiovascular diseases. Recently it was discovered that human adipocytes might be a new target for the immunodeficiency virus-1⁵.

Tanti, J-F. et al., Endocrinology 138(5):2005 (1997)
Okada, T. et al., J. Biochem Mol Biol 269(5):3568 (1994)
Nam, S.Y. et al., Obes Rev 1(2):73 (2000)
Hamilton, B.S. et al, Nature Medicine 1(9):953 (1995)
van Harlemen, V. et al., Diabetes 51:2029 (2002)
Hazan, U. et al., FASEB J. 16(10):1254 (2002)

Characterization: Positive oil droplet accumulation.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat. No.
HPAd Total Kit adult heart (h) subcutaneous(s)	Each kit contains an ampoule of cryopreserved HPAd (802-05), 500 ml of Preadipocyte Growth Medium (811-500), and a Subculture Reagent Kit.	1 Kit	802hK-05ha 802sK-05a
Cryopreserved HPAd adult heart (h) subcutaneous(s)	(>500,000 cells in an ampoule) Cryopreserved at second passage in Preadipocyte Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	802h-05a 802s-05a
Proliferating HPAd adult In Flasks subcutaneous(s)	Cultured in flasks at third passage in Preadipocyte Growth Medium and shipped in Preadipocyte Transfer Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	803s-25a 803s-50a 803s-75a
Proliferating HPAd adult In Flasks heart(h)	Cultured in flasks at third passage in Preadipocyte Growth Medium and shipped in Preadipocyte Transfer Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	803h-25a 803h-50a 803h-75a
Proliferating HPAd adult In Multiwell Plates subcutaneous(s)	Cultured in plates at third passage in Preadipocyte Growth Medium and shipped in Preadipocyte Transfer Medium.	6 well 12 well 24 well 48 well 96 well	803s-6Wa 803s-12Wa 803s-24Wa 803s-48Wa 803s-96Wa
Proliferating HPAd adult In Multiwell Plates heart(h)	Cultured in plates at third passage in Preadipocyte Growth Medium and shipped in Preadipocyte Transfer Medium.	6 well 12 well 24 well 48 well 96 well	803h-6Wa 803h-12Wa 803h-24Wa 803h-48Wa 803h-96Wa
Preadipocyte Growth Medium	Optimized & ready for use	500 ml	811-500
Preadipocyte Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	811K-500
Preadipocyte Basal Medium	Add Growth Supplement before use	500 ml	810-500
Preadipocyte Growth Supplement		50 ml	811-GS
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
HPAd RNA subcutaneous(s)	Total RNA prepared from HPAd	10 µg 25 µg	802s-R10a 802s-R25a