Human Pulmonary Artery Smooth Muscle Cells

(HPASMC)

Human Pulmonary Artery Smooth Muscle Cells (HPASMC) are derived from tunica intima and tunica media of normal human, fibrous plaque-free pulmonary arteries. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. HPASMC are well suited for the study of pulmonary vascular disorders¹, associated liver diseases², and connective tissue diseases³. Phenotypic changes of smooth muscle cells have been found to be related to smooth muscle cell migration and intimal proliferation. Switches in smooth muscle cell phenotype lead to pulmonary hypertension and eventually heart and lung disorders⁴.

Rabinovitch, M., Moss’ Heart Disease in Children, Infants and Adolescents, Williams and Wilkins, Baltimore, p. 856 (1989).
Levine, O.R. et al, J. Pediatr. 83:964 (1973).
Nair, S.S. et al, Arch. Intern. Med. 140:109 (1980).
Rabinovitch, M., Toxicologic Pathology. Vol. 19, No. 4 (Pt.1) pp. 458-468 (1991).

HPASMC

α-actin stain

Characterization: Positive for smooth muscle cell specific a -actin expression.

Each lot is tested negative for HIV, Hepatitis B, Hepatitis C, and negative for mycoplasma, bacteria, yeast and fungi.

Product Name	Product Description	Size (vol)	Cat. No.
HPASMC Total Kit, adult	Each kit contains an ampoule of cryopreserved HPASMC (352-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit.	1 Kit	352K-05a
Cryopreserved HPASMC, adult	(>500,000 cells in an ampoule) Cryopreserved at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	352-05a
Proliferating HPASMC, adult In Flasks	Cultured and shipped in flasks at third passage in Smooth Muscle Cell Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	353-25a 353-50a 353-75a
Proliferating HPASMC, adult In Multiwell Plates	Cultured and shipped in plates at third passage in Smooth Muscle Cell Growth Medium.	6 well 12 well 24 well 48 well 96 well	353-6Wa 353-12Wa 353-24Wa 353-48Wa 353-96Wa
Smooth Muscle Cell Growth Medium	Optimized and ready for use	500 ml	311-500
Smooth Muscle Cell Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	311K-500
Smooth Muscle Cell Basal Medium	Add Growth Supplement before use	500 ml	310-500
Smooth Muscle Cell Growth Supplement		30 ml	311-GS
Smooth Muscle Cell Differentiation Medium		250 ml	311D-250
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
HPASMC RNA, adult	Total RNA prepared from HPASMC	10 µg 25 µg	352-R10a 352-R25a