Human Osteoblasts

(HOb)

Human Osteoblasts (HOb) are isolated from normal human bone. They are cryopreserved at second passage and can be cultured and propagated for at least 10 population doublings. HOb sythesizes bone matrix. Matrix mineralization can be induced in the presence of ascorbic acid and B-Glycerophosphate. These cells are commonly used for micro- and macroscopic investigations of the skeletal system, such as the effects of inflammatory mediators,¹ the effects of bone morphogenic proteins,² the physiological control of bone remodeling,³ the regulation of bone metabolism,⁴ and the study of the chemicals associated with physical trauma.⁵

Frost, A. et al, Acta. Orthop. Scand. 70(5):497-503 (1999)
Takahashi, K., Nippon Yakyurigaku Zasshi. 116(4):232-240 (2000)
Cornish, J. et al Bone 17(4 Suppl):249S-254S (1995)
Malpe, R. et al, J. Bone Miner. Res. 12(3):423-430 (1997)
Reiff. D.A. et al, J. Trauma 50(3):433-438 (2001)

HOb

HOb von Kossa Stained

Characterization: Positive for bone mineralization.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat. No.
HOb Total Kit adult fetal	Each kit contains an ampoule of cryopreserved HOb (406-05), 500 ml of Osteoblast Growth Medium (417-500), a Subculture Reagent Kit (090K).	1 Kit	406K-05a 406K-05f
Cryopreserved HOb adult fetal	(>500,000 cells in an ampoule) Cryopreserved at second passage in Osteoblast Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	406-05a 406-05f
Proliferating HOb In Flasks adult	Cultured in flasks at third passage in Osteoblast Growth Medium and shipped in Osteoblast Transfer Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	407-25a 407-50a 407-75a
Proliferating HOb In Flasks fetal	Cultured in flasks at third passage in Osteoblast Growth Medium and shipped in Osteoblast Transfer Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	407-25f 407-50f 407-75f
Proliferating HOb In Multiwell Plates adult	Cultured in plates at third passage in Osteoblast Growth Medium and shipped in Osteoblast Transfer Medium.	6 well 12 well 24 well 48 well 96 well	407-6Wa 407-12Wa 407-24Wa 407-48Wa 407-96Wa
Proliferating HOb In Multiwell Plates fetal	Cultured in plates at third passage in Osteoblast Growth Medium and shipped in Osteoblast Transfer Medium.	6 well 12 well 24 well 48 well 96 well	407-6Wf 407-12Wf 407-24Wf 407-48Wf 407-96Wf
Osteoblast Growth Medium	Optimized and ready for use.	500 ml	417-500
Osteoblast Growth Medium Kit	Basal Medium & Growth Supplement packaged separately.	Yields 500 ml	417K-500
Osteoblast Basal Medium	Add Growth Supplement before use.	500 ml	416-500
Osteoblast Growth Supplement		50 ml	417-GS
Osteoblast Differentiation Medium		250 ml	417D-250
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
HOb RNA , fetal Differentiated HOb RNA , fetal Stimulated HOb Total RNA , fetal	Total RNA prepared from HOb	10 µg	406-R10f 406D-R10f 406S-R10f
HOb RNA , fetal Differentiated HOb RNA , fetal Stimulated HOb Total RNA , fetal	Total RNA prepared from HOb	25 µg	406-R25f 406D-R25f 406S-R25f