Human Mammary Epithelial Cells

(HMEpC)

Human Mammary Epithelial Cells (HMEpC) are derived from normal adult mammary glands. They are cryopreserved at fifth passage. HMEpC can be cultured and propagated 16 population doublings. It was shown that normal HMEpC have a high amount of cyclin-dependent kinase 6 activity, which restrains HMEpC proliferation, and loss or down-regulation of cdk 6 may play a role in breast tumor development¹. Transforming HMEpC to immortality and malignancy was a useful model for studying early breast carcinogenesis². Autocrine colony-stimulating factor receptors may contribute to invasive progression in epithelial cancer was demonstrated in a 3-dimensional culture of HMEpC³.

Lucas, J.J. et al, Mol. Cancer Res. 2(2):105 (2004).
Stampfer, M.R. et al, J. Mammary Gland Biol. Neoplasia 5(4):365 (2000).
Wrobel, C.N. et al, J. Cell Bio.165(2):263 (2004).

Characterization: Typical epithelial cell morphology.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Name	Product Description	Size (vol)	Cat. No.
HMEpC Total Kit, adult	Each kit contains an ampoule of cryopreserved HMEpC (830-05a), 500 ml of Mammary Epithelial Cell Growth Medium (815-500), and a Subculture Reagent Kit (090K).	1 Kit	830K-05a
Cryopreserved HMEpC , adult	(>500,000 cells in an ampoule) Cryopreserved in ampoules at fifth passage in Mammary Epithelial Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	830-05a
Proliferating HMEpC , adult In Flasks	Cultured and shipped in flasks at sixth passage in Mammary Epithelial Cell Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	831-25a 831-50a 831-75a
Proliferating HMEpC , adult In Multiwell Plates Proliferating HMEpC seeded in inserts are available upon request.	Cultured and shipped in flasks at sixth passage in Mammary Epithelial Cell Growth Medium.	6 well 12 well 24 well 48 well 96 well	831-6Wa 831-12Wa 831-24Wa 831-48Wa 831-96Wa
Mammary Epithelial Cell Growth Medium	Optimized and ready for use	500 ml	815-500
Mammary Epithelial Cell Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	815K-500
Mammary Epithelial Cell Basal Medium	Add Growth Supplement before use	500 ml	814-500
Mammary Epithelial Cell Growth Supplement		5 ml	815-GS
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
HMEpC RNA , adult	Total RNA prepared from HMEpC	10 µg 25 µg	830-R10a 830-R25a