Human Internal Thoracic Artery Smooth Muscle Cells, HITASMC

Human Internal Thoracic (Mammary) Artery

Smooth Muscle Cells

(HITASMC)

Human intrathoratic artery smooth muscle cells (HITASMC) are derived from the intrathoratic artery. They are cryopreserved at second passage. HITASMC can be cultured and propagated 8 population doublings. Proliferation, migration and invasion of SMC are essential pathogenic processes in the development of a broad spectrum of cardiovascular disorders. A Boyden chamber was employed to characterize the diverging migratory and invasion potential of proliferating and nonproliferating human artery SMCs. The differential gene expression of both phenotypes was analyzed by a cDNA array system. Several candidate genes were identified¹. Left internal mammary arteries synthesize endothelium-derived hyperpolarizing factor (EDHF), a short-lived K(+) channel activator that persists after inhibition of nitric oxide (NO) and prostaglandin synthesis. The identity of EDHF is identified as 11,12-epoxyeicosatrienoic acid (11,12-EET) produced by an EDHF synthase CYP450-2C, and accounting for about 40% net endothelial cell relaxation². Expression of non-degradable IkappaB-alpha might have therapeutic potential in both vascular inflammatory reaction and SMC proliferation³.Using HITSMC, it was found that amolodipine can alter Ca2+ mobilization and inhibit voltage-dependent Ca2+ influx and the resulting ERK 1/2 activation⁴. In human vascular smooth muscle cells of both saphenous vein and internal thoracic artery, UTP was found to act as an anti-proliferative regulator⁵.

Blindt R, et al. J. Vasc. Res. 2002 Jul; 39(4): 340-52.
Archer SL, et al. Circulation. 2003 Feb 11; 107(5): 769-76.
Wang Z, et al. J Surg. Res. 2002 Feb; 102(2):198-206.
Stepien O, et al. J. Hypertens.2002 Jan; 20(1):95-102.
White PJ, et al. Am J Physiol Heart Circ Physiol. 2000 Dec; 279(6): H2735-42.

HITASMC

Characterization: Positive for smooth muscle cell specific a -actin expression.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat. No.
HITASMC Total Kit adult	Each kit contains an ampoule of cryopreserved HITASMC (358-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit.	1 Kit	358K-05a
Cryopreserved HITASMC , adult	(>500,000 cells in an ampoule) Cryopreserved at second passage in Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	358-05a
Proliferating HITASMC , adult In Flasks	Cultured and shipped in flasks at third passage in Smooth Muscle Cell Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	359-25a 359-50a 359-75a
Proliferating HITASMC , adult In Multiwell Plates	Cultured and shipped in plates at third passage in Smooth Muscle Cell Growth Medium.	6 well 12 well 24 well 48 well 96 well	359-6Wa 359-12Wa 359-24Wa 359-48Wa 359-96Wa
Smooth Muscle Cell Growth Medium	Optimized and ready for use .	500 ml	311-500
Smooth Muscle Cell Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	311K-500
Smooth Muscle Cell Basal Medium	Add Growth Supplement before use	500 ml	310-500
Smooth Muscle Cell Growth Supplement		30 ml	311-GS
Smooth Muscle Cell Differentiation Medium		250 ml	311D-250
Subculture Reagent Kit	(100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution)	100 ml	090K
HITASMC RNA , adult	Total RNA prepared from HITASMC	10 µg 25 µg	358-R10a 358-R25a