Human Internal Thoracic Artery Endothelial Cells, HITAEC

Human Internal Thoracic (Mammary) Artery Endothelial Cells

(HITAEC)

Human Internal Thoracic (Mammary) Artery Endothelial Cells (HITAEC) are derived from normal human internal thoracic arteries. They are cryopreserved at second passage and can be cultured and propagated at least 10 population doublings.

Study showed that the loss of endothelium-dependent relaxation could dangerously predispose the ITA graft to vasospasm in the post-operative period¹. Increased incidence of VCAM-1 and ICAM-1 in the aorta and ITA may partly explain the enhanced atherosclerosis associated with diabetes mellitus². Contractile responses to angiotensin I and II in the radial artery and internal thoracic artery (ITA) segments were examined in vitro³. Acetylcholine induces endothelium-dependent relaxation of the perforating branch of the ITA by stimulation of mucarinic receptors on the endothelial cells⁴.

Schyvens, C.G. et al, Clin. Exp. Pharmacol. Physiol. 24(3-4):223-8 (1997).
Ribau, J.C. et al, Surg. Res. 85(2):225:33 (1999).
Borland, J.A. et al, Ann. Thorac. Surg. 70(6):2054-63 (2000).
Pesic, S. et al, Pharmacology 63(3):185-90 (2001).

Internal Thoracic Artery Endothelial Cells, HITAEC

Characterization: Positive for factor VIII-related antigen expression. Positive for Dil-Ac-LDL uptake.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.

Product Name	Product Description	Size (vol)	Cat. No.
HITAEC Total Kit , adult	Each kit contains an ampoule of cryopreserved HITAEC (308-05a), 500 ml of HITAEC Growth Medium (212-500), and a Subculture Reagent Kit (090k).	1 Kit	308K-05a
Cryopreserved HITAEC , adult	(>500,000 cells in an ampoule) Cryopreserved at second passage in Endothelial Basal Medium that contains 10% FBS and 10% DMSO.	1 Ampoule	308-05a
Proliferating HITAEC , adult In Flasks	Cultured and shipped in flasks at third passage in HITAEC Growth Medium.	T-25 Flask 2 T-25 Flasks T-75 Flask or 3 T-25 Flasks	309-25a 309-50a 309-75a
Proliferating HITAEC , adult In Multiwell Plates	Cultured and shipped in plates at third passage in HITAEC Growth Medium.	6 well 12 well 24 well 48 well 96 well	309-6Wa 309-12Wa 309-24Wa 309-48Wa 309-96Wa
HITAEC Growth Medium	Optimized and ready for use	500 ml	212-500
HITAEC Growth Medium Kit	Basal Medium & Growth Supplement packaged separately	Yields 500 ml	212K-500
HITAEC Basal Medium	Add Growth Supplement before use	500 ml	210-500
HITAEC Growth Supplement		30 ml	212-GS
Subculture Reagent Kit	100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution	100 ml	090K
HITAEC RNA , adult	Total RNA prepared from HITAEC	10 µg	308-R10a
HITAEC RNA , adult	Total RNA prepared from HITAEC	25 µg	308-R25a